Objective To explore the roles of CyclinD1 and P16 proteins in the oncogenesis of nephroblastoma and the relationship between these two protein production and the pathological characteristics of nephroblastoma. Methods Immunohistochemical technique was applied to detect the expression of CyclinD1 and P16 in 36 cases of nephroblastoma and 8 cases of normal nephritic tissues. Results (1) The expression of cyclinD1 and P16 in the nephroblastoma tissues is 55.56%(20/36),and 61.11%(22/36), respectively; There was a significant difference in the CyclinDl between the nephroblastoma tissue and normal tissue(P < 0.05), no significant difference in the P16 between the nephroblastoma tissue and normal tissue(P>0.05) (2) There were no significant difference in the CyclinD1 and P16 protein about the histological typing ,clinical stage, lymph node metastases , respectively (P > 0.05); (3) There was no correlation between the expression of CyclinD1 and P16 (r(s) =-0.025, P > 0.05). Conclusions (1)The over-expression of cyclinD1 is highly related to the oncogenesis of nephroblastoma.(2)Cytoplasmic staining patterns of cyclinD1 and P16 positive cell may be associate with the occurrence of nephroblastoma. |