SIRPA在哮喘中的表达及其预警价值的研究-现代医学

SIRPA在哮喘中的表达及其预警价值的研究

单位：1. 遵义市妇幼保健院, 贵州遵义 563099;
2. 贵州省儿童医院/遵义医科大学附属医院儿科内科, 贵州遵义 563000;
3. 重庆医科大学, 重庆 400016;
4. 遵义医科大学实验动物中心, 贵州遵义 563000;
5. 遵义医科大学附属医院医学检验科, 贵州遵义 563000;
6.

关键词：哮喘 SIRPA 生信分析预警机制

分类号：R562.25

出版年·卷·期（页码）：2025·53·第八期（1205-1210）

摘要：

目的：探究信号调控蛋白A(SIRPA)在哮喘中的表达及促进哮喘发生的预警价值，并探讨其调节哮喘发生的通路机制。方法:采用哮喘患者SIRPA表达及受试者工作特征(ROC)曲线分析SIRPA与哮喘发生的相关性；Western Blot和免疫荧光检测哮喘动物肺组织SIRPA基因蛋白水平；免疫组织化学分析哮喘动物肺组织中SIRPA的表达；基因集富集分析探究哮喘患者SIRPA调节哮喘发生的机制；皮尔森系数分析哮喘患者SIRPA高表达与免疫细胞富集的相关性。结果：与健康人群组相比, 哮喘患者外周血SIRPA的表达显著增加(P＜0.05)；哮喘患者SIRPA表达量ROC曲线下面积(AUC)=0.72，SIRPA高表达与哮喘发生呈正相关，差异有统计学意义(P＜0.05)；与康复人群组相比, 哮喘患者SIRPA表达显著增加(P＜0.05)；哮喘动物发现肺组织中SIRPA的蛋白水平显著增加(P＜0.001)；哮喘患者SIRPA 通过破骨细胞分化信号通路调节哮喘发生；哮喘患者SIRPA高表达与中性粒细胞的富集呈正相关，差异有统计学意义(P＜0.01)，与CD4 记忆T细胞呈负相关，差异有统计学意义(P＜0.01)。结论：SIRPA高表达与哮喘发病相关，其发病通路可能与破骨细胞分化调控、中性粒细胞增加及CD4记忆T细胞减少有关，对哮喘诊断预警具有一定价值。

Objective: To explore the expression of signal-regulated protein alpha(SIRPA) in asthma and its early warning value in asthma occurrence, and to explore the pathway mechanism of its regulation of its occurrence. Methods: The correlation between SIRPA and asthma pathogenesis was analyzed by SIRPA expression and the receiver operator characteristic(ROC) curves. Western blot and immunofluorescence were used to detect the protein level of SIRPA gene in lung tissues of an asthma model. Immunohistochemistry was adopted to analyze the expression of SIRPA in asthma. Gene set enrichment analysis was employed to explore the mechanism of SIRPA in regulating asthma pathogenesis. Pearson's coefficient was used to analyze the correlation between the high expression of SIRPA and the enrichment of immune cells. Results: The expression of SIRPA in the peripheral blood of asthma patients was significantly increased compared with that of healthy subjects(P<0.05). The area under the ROC curve(AUC) of the expression of SIRPA in asthma patients was 0.72, and high expression of SIRPA was positively correlated with the development of asthma, with a statistically significant difference(P<0.05). Compared with the recovered population group, the expression of SIRPA in asthma patients was significantly increased(P<0.05). The asthma model revealed a significant increase in the protein level of SIRPA in lung tissues(P<0.001). SIRPA regulated the development of asthma through the osteoclast differentiation signaling pathway. The high expression of SIRPA in asthma was positively correlated with the enrichment of neutrophils and negatively correlated with the CD4 memory T-cells with a statistically significant difference(P<0.01). Conclusion: The high expression of SIRPA is associated with the development of asthma, and the pathway of its pathogenesis may be related to the regulation of osteoclast differentiation, the increase of neutrophils and the decrease of CD4 memory T-cells, which is valuable for the early warning of asthma diagnosis.

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