KCNK5蛋白在肺腺癌中的表达及临床意义-现代医学

KCNK5蛋白在肺腺癌中的表达及临床意义

作者：李源丽¹ 韩连奎² 3 4 杨迎春² 3 4 马雪¹ 童英² 3 4 朱海振² 3 4

单位：1. 贵州中医药大学研究生院, 贵州贵阳 550002;
2. 贵州省人民医院肿瘤科, 贵州贵阳 550002;
3. 贵州省人民医院肺脏免疫性疾病诊治疗国家重点实验室, 贵州贵阳 550002;
4. 贵州省癌症中心, 贵州贵阳 550002

关键词：肺腺癌 KCNK5蛋白免疫组织化学预后

分类号：R734.2

出版年·卷·期（页码）：2025·53·第二期（211-217）

摘要：

目的：旨在探究KCNK5蛋白在肺腺癌(LUAD)组织中的表达水平及其与临床病理特征、预后之间的关系。方法：收集70例LUAD患者的癌组织及其癌旁组织(距肿瘤边缘5 cm处)标本，通过对LUAD组织的生物学信息研究及免疫组织方法检验LUAD组织及癌旁正常组织中KCNK5蛋白表达，分析其与LUAD临床病理特征的关系，采用单因素和多因素COX回归法分析影响LUAD患者预后的因素。结果：免疫组化结果显示，KCNK5蛋白表达于LUAD细胞质，癌旁正常组织呈阴性或弱阳性;生物学信息分析得出LUAD组织KCNK5 mRNA表达均高于正常肺组织(P<0.05)。KCNK5蛋白高表达与LUAD患者的临床分期、淋巴结转移、分化程度有关联(P<0.05);多因素Logistic分析显示KCNK5蛋白高表达与淋巴结转移是影响肺腺癌预后的危险因素(P<0.05)。结论： KCNK5蛋白与LUAD患者淋巴结转移和预后密切相关，KCNK5蛋白有望成为肺腺癌早期诊断标志物及评估预后的指标。

Objective: To investigate the expression level of KCNK5 protein in lung adenocarcinoma(LUAD) tissues and the association between clinicopathological features and patient prognosis. Methods: Cancer tissue specimens from 70 LUAD patients and their paracancerous tissues(5 cm from the tumor margin) were collected. The biological information of LUAD tissues and the expression of KCNK5 protein in LUAD tissues and normal tissues adjacent to the cancer were examined by immunohistochemistry to analyze the connection between KCNK5 protein expression and the clinicopathological characteristics of LUAD, and the prognostic factors of LUAD were analyzed by binary and multiple regression methods. Results: The immunohistochemical results showed that KCNK5 protein was expressed in the cytoplasm of LUAD cells, and the normal tissues next to the cancer were negative or weakly positive; biological information analysis showed that the expression of KCNK5 mRNA in LUAD tissues was higher than that in the normal lung tissues(P<0.05); the expression of KCNK5 protein was closely correlated with the clinical stage, lymph node metastasis, and the degree of differentiation of the patients with LUAD(P<0.05); multifactorial logistic regression was used to analyze its relation with the clinicopathological features of patients with LUAD. Multifactorial Logistic regression analysis further confirmed that high KCNK5 protein expression and lymph node metastasis were independent risk factors affecting the prognosis of lung adenocarcinoma(P<0.05). Conclusion: KCNK5 protein is closely related to lymph node metastasis and prognosis of LUAD patients, and KCNK5 is expected to be an early diagnostic marker and prognostic indicator.

参考文献：

[1] SIEGEL R L,MILLER K D,WAGLE N S,et al.Cancer statistics,2023[J].CA CancerJ Clin,2023,73(1):17-48.
[2] 齐利青.NUSAP1在肺腺癌中的表达及预后价值研究[D].太原:山西医科大学,2023.
[3] 雷宇,黄山,路燕,等.肺腺癌组织中Trophinin相关蛋白的表达及临床病理联系[J].安徽医科大学学报,2020,55(1):118-123.
[4] JIN C Y,DU L,NUERLAN A H,et al.High expression of RRM2 as an independent predictive factor of poor prognosis in patients with lung adenocarcinoma[J].Aging (Albany NY).2020,13(3):3518-3535.
[5] NESOVIC-OSTOJIC J,MARKOVIC-LIPKOVSKI J,TODOROVIC J,et al.Immunolocalization of the TASK2 Potassium channel in fry[J].Folia Biol (Krakow),2016,64(3):183-188.
[6] NEMATIAN-ARDESTANI E,ABD-WAHAB F,CHATELAIN F C,et al.Selectivity filter instability dominates the low intrinsic activity of the TWIK-1 K2P K⁺ channel[J].J Biol Chem,2020,295(2):610-618.
[7] PARDO L A, STVHMER W. The roles of K (+) channels in cancer[J]. Nat Rev Cancer,2014,14(1):39-48.
[8] 薛刚,孙新民,林旭,等.KCNK5双孔钾通道蛋白在甲状腺乳头状癌中的表达及临床意义[J].山东大学耳鼻喉眼学报,2021,35(4):64-69.
[9] ALVAREZ-BARON C P,JONSSON P,THOMAS C,et al.The two-pore domain potassium channel KCNK5:induction by estrogen receptor alpha and role in proliferation of breast cancer cells[J].Mol Endocrinol,2011,25(8):1326-1336.
[10] FONG P,ARGENT B E,GUGGINO W B,et al.Characterization of vectorial chloride transport pathways in the human pancreatic duct adenocarcinoma cell line HPAF[J].Am J Physiol Cell Physiol,2003,285(2):C433-C445.
[11] LUCCHETTA M,DA PIEDADE I,MOUNIR M,et al.Distinct signatures of lung cancer types:aberrant mucin O-glycosylation and compromised immune response[J].BMC Cancer,2019,19(1):824.
[12] TAN F,BI N,ZHANG H,et al.External validation of the eighth edition of the TNM classification for lung cancer in small cell lung cancer[J].Lung Cancer,2022,170:98-104.
[13] DENG X,XIONG W,JIANG X,et al.LncRNA LINC00472 regulates cell stiffness and inhibits the migration and invasion of lung adenocarcinoma by binding to YBX1[J].Cell Death Dis,2020,11(11):945.
[14] DONG Y,ZHOU L,ZHAO D,et al.MUC5AC enhances tumor heterogeneity in lung adenocarcinoma with mucin production and is associated with poor prognosis[J].Jpn J Clin Oncol,2020,50(6):701-711.
[15] REYES R,DUPRAT F,LESAGE F,et al.Cloning and expression of a novel pH-sensitive two pore domain K⁺ channel from human kidney[J].J Biol Chem,1998,273(47):30863-30869.
[16] NIEMEYER M I,CID L P,GONZÁLEZ W,et al.Gating,regulation,and structure in K2P K⁺ channels:in varietate concordia?[J].Mol Pharmacol,2016,90(3):309-317.
[17] BUSTOS D,BEDOYA M,RAMÍREZ D,et al.Elucidating the structural basis of the intracellular pH sensing mechanism of TASK-2 K2P channels[J].Int J Mol Sci,2020,21(2):532.
[18] ZHAO K Q,XIONG G,WILBER M,et al.A role for two-pore K⁺ channels in modulating Na⁺ absorption and Cl^- secretion in normal human bronchial epithelial cells[J].Am J Physiol Lung Cell Mol Physiol,2012,302(1):L4-L12.
[19] ZÚÑIGA L,CAYO A,GONZÁLEZ W,et al.Potassium channels as a target for cancer therapy:current perspectivess[J].Onco Targets Ther,2022,15:783-797.
[20] DOOKERAN K A,ZHANG W,STAYNER L,et al.Associations of two-pore domain potassium channels and triple negative breast cancer subtype in The Cancer Genome Atlas:systematic evaluation of gene expression and methylation[J].BMC Res Notes,2017,10(1):475.
[21] LI W C,XIONG Z Y,HUANG P Z,et al.KCNK levels are prognostic and diagnostic markers for hepatocellular carcinoma[J].Aging (Albany NY),2019,11(19):8169-8182.
[22] SANTARIUS T,BIGNELL G R,GREENMAN C D,et al.GLO1-a novel amplified gene in human cancer[J].Genes Chromosomes Cancer,2010,49(8):711-725.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录