松果菊苷调节CXCL12/CXCR4信号通路对牙髓干细胞增殖、迁移和分化的影响-现代医学

松果菊苷调节CXCL12/CXCR4信号通路对牙髓干细胞增殖、迁移和分化的影响

单位：邯郸市口腔医院牙体牙髓一科, 河北邯郸 056001

分类号：R781.3

出版年·卷·期（页码）：2024·52·第十一期（1688-1695）

摘要：

目的: 探讨松果菊苷(ECH)调节CXC趋化因子配体12(CXCL12)/CXC趋化因子受体4(CXCR4)信号通路对牙髓干细胞(DPSCs)增殖、迁移和分化的影响。方法: 以DPSCs为研究对象，分别经不同浓度(0.01 mg·mL^-1、0.1 mg·mL^-1、1 mg·mL^-1)的ECH处理，记为ECH低浓度(ECH-L)组、ECH中浓度(ECH-M)组、ECH高浓度(ECH-H)组；以1 mg·mL^-1ECH、100 nmol·L^-1 AMD3100同时处理DPSCs，记为ECH-H+AMD3100组，并以未经处理的DPSCs为空白对照组；MTT法检测细胞增殖；Transwell实验检测细胞迁移；Western blot检测CXCL12、CXCR4蛋白表达；成骨诱导21 d后，茜素红S染色评估矿化结节形成；碱性磷酸酶(ALP)试剂盒分析ALP活性；qRT-PCR检测牙本质基质蛋白1(DMP1)、骨钙素(OCN)、ALP mRNA表达水平。结果: 与空白对照组相比，ECH-L组、ECH-M组、ECH-H组CXCL12、CXCR4蛋白表达、迁移数、ALP含量、矿化结节含量、A₄₅₀值、DMP1、OCN、ALP mRNA表达水平显著增加，不同浓度ECH间均具有统计学差异(P<0.05)；与ECH-H组相比，ECH-H+AMD3100组CXCL12、CXCR4蛋白表达、迁移数、ALP含量、矿化结节含量、A₄₅₀值、DMP1、OCN、ALP mRNA表达水平显著降低(P<0.05)。结论: ECH通过上调CXCL12/CXCR4信号通路促进DPSCs增殖、迁移和分化。

Objective: To investigate the impacts of Echinacetin(ECH) on the proliferation, migration, and differentiation of dental pulp stem cells(DPSCs) by regulating the CXC chemokine ligand 12(CXCL12)/CXCR4 signaling pathway. Methods: DPSCs were studied and subjected to different concentrations of ECH(0.01 mg·mL^-1, 0.1 mg·mL^-1, 1 mg·mL^-1), and they were grouped into low ECH concentration(ECH-L) group, medium ECH concentration(ECH-M) group, and high ECH concentration(ECH-H) group; DPSCs were treated with 1 mg·mL^-1 ECH and 100 nmol·L^-1 AMD3100 simultaneously, and recorded as the ECH-H+AMD3100 group, and untreated DPSCs were used as blank control group; MTT method was applied to detect cell proliferation; Transwell experiment was applied to detect cell migration; Western blot was applied to detect the expression of CXCL12 and CXCR4 proteins; 21 days after osteogenic induction, alizarin red S staining was applied to evaluate the formation of mineralized nodules; alkaline phosphatase(ALP) assay kit was applied to analyze ALP activity; qRT-PCR was applied to detect the expression levels of dentin matrix protein 1(DMP1), osteocalcin(OCN), and ALP mRNA. Results: Compared with the blank control group, the expression of CXCL12, CXCR4 protein, migration number, ALP content, mineralized nodule content, A₄₅₀value, expression levels of DMP1, OCN, and ALP mRNA in the ECH-L group, ECH-M group, and ECH-H group obviously increased, there were statistical differences among different concentrations of ECH(P<0.05); compared with the ECH-H group, the expression of CXCL12, CXCR4 protein, migration number, ALP content, mineralized nodule content, A₄₅₀value, expression levels of DMP1, OCN, and ALP mRNA in the ECH-H+AMD3100 group obviously decreased(P<0.05). Conclusion: ECH promotes the proliferation, migration, and differentiation of DPSCs by up-regulating the CXCL12/CXCR4 signaling pathway.

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