基于双硫死亡相关lncRNA的宫颈癌预后模型构建及验证-现代医学

基于双硫死亡相关lncRNA的宫颈癌预后模型构建及验证

单位：1. 南华大学附属第二医院肿瘤一区, 湖南衡阳 421000;
2. 南华大学附属第二医院病理科, 湖南衡阳 421000

分类号：R737.33; R363

出版年·卷·期（页码）：2024·52·第二期（181-187）

摘要：

目的：构建预测宫颈癌生存期的双硫死亡相关长链非编码RNA(lncRNA)预后模型。方法：从TCGA数据库下载307个宫颈癌RNA测序数据及临床信息，随机分为训练集和测试集。使用共表达分析和回归分析得到双硫死亡相关lncRNA并构建预后模型。根据风险评分中位数将患者分为高风险组和低风险组。使用Kaplan-Meier生存分析、Cox回归分析、受试者工作特征(ROC)曲线、主成分分析、列线图和校准曲线评估模型的预测效能。结果：共得到3个双硫死亡相关lncRNA并构建预后模型。Kaplan-Meier生存分析结果显示高风险组的总生存期短于低风险组。风险曲线显示高危患者的死亡率高于低危患者。Cox回归分析显示风险评分是独立预后因子。该模型预测患者1年、3年、5年存活率的ROC曲线下面积(AUC)分别为 0.780、0.701、0.716。列线图和校准曲线表明预后模型接近于理想模型。结论：本研究构建了宫颈癌的双硫死亡相关lncRNA预后模型，该模型预测效能良好。

Objective: To construct a prognostic model of disulfidptosis-related long non-coding RNA(lncRNA) for predicting the survival of cervical cancer. Methods: 307 cervical cancer RNA sequencing data and clinical information were downloaded from TCGA database and randomly divided into training and test set. The disulfidptosis-related lncRNA were obtained by co-expression analysis and regression analysis, and a prognostic model was constructed. Patients were divided into high risk group and low risk group based on median risk score. Finally, Kaplan-Meier survival analysis, Cox regression analysis, receiver operator characteristic curves, principal component analysis, calibration curve evaluation model were used to evaluate the prediction efficiency of the model. Results: A total of 3 disulfidptosis-related lncRNA were obtained and prognosis models were constructed. Kaplan-Meier survival analysis showed that the overall survival of the high-risk group was shorter than that of the low-risk group，the mortality rate was higher than low-risk patients. Cox regression analysis showed that risk score was an independent prognostic factor. The AUC of survival rates for 1-year, 3-year and 5-year were 0.780, 0.701 and 0.716. The principal component analysis and calibration curve evaluation showed that the prediction model was close to the idealized model. Conclusion: A prognostic model of disulfidptosis-related lncRNA in cervical cancer is constructed, which is effective.

参考文献：

[1] FENG X,SONG Z,TAO A,et al.Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study[J].Front Nutr,2023,10:1136590.
[2] 龚敏,刘巧玲.高危型HPV分型定量与宫颈癌相关病变关系研究[J].现代医学,2022，50(4):460-465.
[3] LIU X,NIE L,ZHANG Y,et al.Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis[J].Nat Cell Biol,2023,25(3):404-414.
[4] WANG H.MicroRNAs and apoptosis in colorectal cancer[J].Int J Mol Sci,2020,21(15):5353.
[5] MERCER T R,DINGER M E,MATTICK J S.Long non-coding RNAs:insights into functions[J].Nat Rev Genet,2009,10(3):155-159.
[6] XIA C,DONG X,LI H,et al.Cancer statistics in China and United States,2022:profiles,trends,and determinants[J].Chin Med J(Engl),2022,135(5):584-590.
[7] PFAENDLER K S,TEWARI K S.Changing paradigms in the systemic treatment of advanced cervical cancer[J].Am J Obstet Gynecol,2016,214(1):22-30.
[8] SIEGEL R L,MILLER K D,JEMAL A.Cancer statistics,2018[J].CA Cancer J Clin,2018,68(1):7-30.
[9] FERRALL L,LIN K Y,RODEN R,et al.Cervical cancer immunotherapy:facts and hopes[J].Clin Cancer Res,2021,27(18):4953-4973.
[10] LI J,CAO F,YIN H L,et al.Ferroptosis:past,present and future[J].Cell Death Dis,2020,11(2):88.
[11] TSVETKOV P,COY S,PETROVA B,et al.Copper induces cell death by targeting lipoylated TCA cycle proteins[J].Science,2022,375(6586):1254-1261.
[12] WINKLE M,EL-DALY S M,FABBRI M,et al.Noncoding RNA therapeutics-challenges and potential solutions[J].Nat Rev Drug Discov,2021,20(8):629-651.
[13] YANG Q,AL-HENDY A.The regulatory functions and the mechanisms of long non-coding rnas in cervical cancer[J].Cells,2022,11(7):1149.
[14] LIU Z,MO H,SUN L,et al.Long noncoding RNA PICSAR/miR-588/EIF6 axis regulates tumorigenesis of hepatocellular carcinoma by activating PI3K/AKT/mTOR signaling pathway[J].Cancer Sci,2020,111(11):4118-4128.
[15] LV J,ZHANG W,WANG Y.Long non-coding RNA PICSAR serves as a non-invasive biomarker for the diagnosis and prognosis of cutaneous squamous cell carcinoma[J].Clin Exp Med,2021,21(4):579-586.
[16] LU X,GAN Q,GAN C,et al.Long non-coding RNA PICSAR knockdown inhibits the progression of cutaneous squamous cell carcinoma by regulating miR-125b/YAP1 axis[J].Life Sci,2021,274:118303.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录