视神经萎缩症蛋白1在胃癌组织中的表达及其对胃癌细胞增殖及凋亡的影响-现代医学

视神经萎缩症蛋白1在胃癌组织中的表达及其对胃癌细胞增殖及凋亡的影响

单位：1. 石家庄市人民医院普外科, 河北石家庄 050000;
2. 石家庄市人民医院核医学科, 河北石家庄 050000

分类号：R73-3; R735

出版年·卷·期（页码）：2023·51·第九期（1227-1234）

摘要：

目的：探究视神经萎缩症蛋白1(OPA1)在胃癌(GC)组织中的表达及其对GC细胞增殖及凋亡的影响。方法：应用TCGA和GTEx数据库分析GC组织及癌旁组织中OPA1基因的表达差异。利用qRT-PCR和免疫组织化学检测101例GC组织及癌旁组织中OPA1 mRNA和蛋白表达情况。采用qRT-PCR和Western blot检测人正常胃黏膜上皮细胞GES-1及5株人GC细胞系(AGS、MGC-803、MKN-45、SGC-7901、HGC-27)中OPA1 mRNA和蛋白表达水平。选择OPA1表达量最高的2株GC细胞AGS、SGC-7901为研究对象，将OPA1小干扰RNA(siRNA)慢病毒质粒及其阴性对照分别感染GC细胞，采用荧光显微镜观察感染效率；qRT-PCR检测细胞中OPA1 mRNA表达水平；CCK-8法检测细胞增殖活性；平板克隆形成实验检测细胞克隆形成能力；流式细胞术检测细胞凋亡水平；Western blot法检测细胞中OPA1、Cleaved-caspase-3、Bax和Bcl-2等蛋白表达水平。结果：OPA1基因在GC组织及细胞株中高表达，siRNA靶向沉默OPA1基因表达可显著下调AGS和SGC-7901细胞中OPA1 mRNA和蛋白表达水平，降低细胞增殖活性和克隆形成能力，并诱导细胞凋亡，上调Cleaved-caspase-3、Bax蛋白表达水平，下调Bcl-2蛋白表达水平。结论：OPA1基因在GC组织和细胞中高表达，干扰其表达可抑制GC细胞增殖并诱导细胞凋亡。

Objective: To investigate the expression of optic atrophy protein-1(OPA1) in gastric cancer(GC) and its effect on the proliferation and apoptosis of GC cells. Methods: The TCGA and GTEx database was used to analyze the difference of OPA1 gene expression in GC tissues and adjacent tissues. The expression levels of OPA1 mRNA and protein in 101 GC tissues and adjacent tissues were detected by qRT-PCR and immunohistochemistry. The expression levels of OPA1 mRNA and protein in human normal gastric epithelial cells GES-1 and five human GC cell lines(AGS, MGC-803, MKN-45, SGC-7901 and HGC-27) were detected by qRT-PCR and Western blot. Two cell lines with the highest expression of OPA1, AGS and SGC-7901, were selected as the research objects. OPA1 small interfering RNA(siRNA) lentiviral plasmids and its negative control were respectively infected with GC cells, and the infection efficiency was observed by fluorescence microscope. OPA1 mRNA expression was detected by qRT-PCR. CCK-8 assay was used to detect cell proliferation. The ability of cell clonogenesis was detected by plate clone formation assay. The level of apoptosis was detected by flow cytometry. The expression levels of Cleaved-caspase-3, Bax and Bcl-2 apoptosis-related proteins were detected by Western blot. Results: OPA1 gene was highly expressed in GC tissues and cell lines. SiRNA targeting OPA1 gene significantly down-regulated the mRNA and protein expression levels of OPA1 in gastric cancer AGS and SGC-7901 cells, decreased cell proliferation activity and clone formation ability, induced cell apoptosis, up-regulated the protein expression levels of Cleaved-caspase-3 and Bax, and down-regulated the protein expression level of Bcl-2. Conclusion: OPA1 gene is highly expressed in GC tissues and cells, and interfering with its expression inhibits the proliferation of GC cells and induces apoptosis.

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