Objective: To investigate the expression of miR-125b and miR-1276 in serum of patients with bladder cancer(BC) and their clinical significance. Methods: A total of 114 patients with BC admitted from May 2017 to May 2019 were included into study group(BC group), meanwhile 108 patients who underwent physical examination in Linxi Hospital of Kailuan General Hospital were regarded as control group. The expression levels of miR-125b and miR-1276 in serum of the BC group and the control group and BC patients with different clinical characteristics were compared. The relationship between miR-125b and miR-1276 in serum of BC patients was analyzed to reveal the predictive value of serum miR-125b and miR-1276 for BC occurrence and their relationship with prognosis and survival. The factors influencing the survival status of BC patients were also explored. Results: The levels of serum miR-125b and miR-1276 in BC group were obviously lower than those in the control group(P<0.05).There was no obvious difference in the levels of miR-125b and miR-1276 in BC patients with different age, gender and tumor size(P>0.05), there were obvious differences in serum miR-125b and miR-1276 levels in patients with different TNM stages, lymph node metastasis, pathological grade and depth of invasion(P<0.05). Pearson correlation analysis showed that there was a positive correlation between miR-125b and miR-1276 in BC patients(r=0.683, P<0.001). The area under the curve(AUC) of serum miR-125b and miR-1276 levels for diagnosing BC was 0.909 and 0.911, respectively, sensitivity was 90.4% and 85.1% respectively, and specificity was 74.7% and 81.4% respectively. The AUC of combined diagnosis of BC was 0.963, and its sensitivity and specificity were 94.7% and 83.6% respectively. Logistic regression analysis showed that serum miR-125b and miR-1276 were independent risk factors affecting the survival status of BC patients(P<0.05). The survival rate of high expression of miR-125b and miR-1276 in serum were obviously higher than those of low expression(χ21=17.384, χ22=19.859,P<0.01). Conclusion: The serum levels of miR-125b and miR-1276 in patients with BC are abnormally low, in addition, those with low serum miR-125b and miR-1276 levels have poor survival conditions, therefore, the expression levels of miR-125b and miR-1276 in the serum of BC patients can determine the survival status of patients. |
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