Objective: To explore the effect of miR-150-3p targeting CDH2 gene on lung tissue damage in neonatal rats with hypoxia-induced pulmonary hypertension(HPH). Methods: Newborn Wistar rats were randomly divided into control group, model group, negative control group, and miR-150-3p overexpression group, with 10 rats in each group. Except for the control group, the rest of the groups constructed a newborn rat HPH model. The mean pulmonary arterial pressure(mPAP) of rats in each group was measured when hypoxia for 3, 6, and 12 d. Enzyme-linked immunosorbent assay(ELISA) was used to detect the levels of hypoxia inducible factor-1α(HIF-1α) and endothelin-1(ET-1) in rat serum; on the 12th day of hypoxia, Western blotting was used to detect the expression of HIF-1α, ET-1, iNOS and CDH2 in the lung tissue of rats, RT-PCR was used to detect the expression of miR-150-3p and CDH2 mRNA in the lung tissue of rats. The dual luciferase reporter gene experiment was used to verify the targeting relationship between miR-150-3p and CDH2. Results: Compared with the control group, rats in the model group and the negative control group showed slow response, malaise, reduced appetite, lung tissue inflammatory cell infiltration, and alveolar size changes; mPAP, the levels of serum HIF-1α and ET-1, the expression of HIF-1α, ET-1, iNOS and CDH2 proteins in lung tissue were all significantly increased(P<0.05), the expression of miR-150-3p in lung tissue was significantly reduced, and the expression of CDH2 mRNA was significantly increased(P<0.05). Compared with the model group, there was no significant difference in the above indicators of the negative control group(P>0.05). Compared with the model group and the negative control group, the rats in the miR-150-3p overexpression group showed a good improvement in slow response and inflammatory cell infiltration; mPAP, the levels of serum HIF-1α and ET-1, the expression of HIF-1α, ET-1, iNOS and CDH2 proteins in lung tissue were all significantly decreased(P<0.05), the expression of miR-150-3p in lung tissue was significantly reduced, and the expression of CDH2 mRNA was significantly decreased(P<0.05). The results of the dual luciferase reporter gene experiment proved that CDH2 was the target gene of miR-150-3p. Conclusion: MiR-150-3p can reduce lung tissue damage in newborn rats with HPH by targeting CDH2 gene.
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