circDHRS3通过调节TLR4/NF-κB信号通路对关节软骨细胞增殖和凋亡的影响-现代医学

circDHRS3通过调节TLR4/NF-κB信号通路对关节软骨细胞增殖和凋亡的影响

单位：苏州市中西医结合医院骨科, 江苏苏州 215100

分类号：R684

出版年·卷·期（页码）：2022·50·第八期（934-939）

摘要：

目的：探讨circDHRS3通过调节TLR4/NF-κB信号通路对关节软骨细胞增殖和凋亡的影响。方法：体外分离培养人关节软骨细胞，用不同浓度(0、1、5、10 μg·L^-1)的IL-1β处理，采用qRT-PCR检测circDHRS3的表达。选取10 μg·L^-1处理关节软骨细胞，将细胞分组，分别为Control组、IL-1β组、IL-1β+si-NC组、IL-1β+si-circDHRS3组、IL-1β+si-circDHRS3+pcDNA组和IL-1β+si-circDHRS3+TLR4组。CCK8检测细胞活力，流式细胞术检测细胞凋亡；Weatern blot检测p21、PCNA、Bcl-2、Bax和TLR4/NF-κB信号通路相关蛋白的表达。结果：经过不同浓度IL-1β处理关节软骨细胞后，circDHRS3在软骨细胞中高表达，呈浓度依赖性。与Control组相比，IL-1β组抑制细胞活力及PCNA、Bcl-2蛋白表达，促进细胞凋亡及p21、Bax、TLR4和p-NF-κB蛋白表达；与IL-1β+si-NC组相比，IL-1β+si-circDHRS3组促进细胞活力及PCNA、Bcl-2蛋白表达，抑制细胞凋亡及p21、Bax、TLR4和p-NF-κB蛋白表达；与IL-1β+si-circDHRS3+pcDNA组相比，IL-1β+si-circDHRS3+TLR4组抑制细胞活力及PCNA、Bcl-2蛋白表达，促进细胞凋亡及p21、Bax蛋白表达。结论：circDHRS3通过上调TLR4/NF-κB信号通路抑制关节软骨细胞的增殖和诱导细胞的凋亡。

Objective: To investigate the effect of circDHRS3 on the proliferation and apoptosis of articular chondrocytes by regulating the TLR4/NF-κB signaling pathway.Methods: Human articular chondrocytes were isolated and cultured in vitro,treated with IL-1β at different concentrations (0,1,5,10 μg·L^-1),and the expression of circDHRS3 was detected by qRT-PCR.Select 10 μg·L^-1 to treat articular chondrocytes,and divide the cells into control group,IL-1β group,IL-1β+si-NC group,IL-1β+si-circDHRS3 group,IL-1β+si-circDHRS3+pcDNA group and IL-1β+si-circDHRS3+TLR4 group.Cell viability was detected using CCK8;cell apoptosis was detected with flow cytometry;Weatern blot was used to detect the expression of p21,PCNA,Bcl-2,Bax and TLR4/NF-κB signaling pathway related proteins.Results: After treating articular chondrocytes with different concentrations of IL-1β,circDHRS3 was highly expressed in chondrocytes in a concentration-dependent manner.Compared with the control group,the IL-1β group inhibited cell viability,PCNA,Bcl-2 protein expression;and promoted the rate of cell apoptosis,p21,Bax,TLR4 and p-NF-κB protein expression.Compared with IL-1β+si-NC group,IL-1β+si-circDHRS3 group promoted cell viability,PCNA,Bcl-2 protein expression;inhibited cell apoptosis rate,p21,Bax,TLR4 and p-NF-κB protein expression.Compared with IL-1β+si-circDHRS3+pcDNA group,IL-1β+si-circDHRS3+TLR4 group inhibited cell viability,PCNA,Bcl-2 protein expression;promoted cell apoptosis rate,p21 protein expression,and Bax protein expression significantly elevated.Conclusion: circDHRS3 inhibits the proliferation of articular chondrocytes and induces cell apoptosis by up-regulating the TLR4/NF-κB signaling pathway.

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