血清miR-195-5p、IL-17对小儿支原体肺炎早期诊断价值的研究-现代医学

血清miR-195-5p、IL-17对小儿支原体肺炎早期诊断价值的研究

单位：秦皇岛市第三医院儿科, 河北秦皇岛 066000

分类号：R725.6

出版年·卷·期（页码）：2022·50·第七期（875-879）

摘要：

目的：研究血清miR-195-5p、白细胞介素（IL）-17在支原体肺炎（MPP）患儿早期的水平变化及其诊断价值。方法：选取2018年2月至2019年2月本院儿科呼吸门诊就诊的150例MPP患儿作为MPP组，同期选取50例健康体检儿童作为对照组。采用实时荧光定量PCR（qRT-PCR）法测定两组儿童血清miR-195-5p的表达水平；采用酶联免疫吸附法（ELISA）检测两组儿童血清IL-17的水平。比较两组血清miR-195-5p、IL-17水平；采用Pearson法分析血清miR-195-5p、IL-17水平的相关性；绘制受试者工作特征（ROC）曲线评估血清miR-195-5p、IL-17水平及两者联合检测对MPP的早期预测价值；采用Logistic回归分析MPP发生的影响因素。结果：与对照组比较，MPP组患儿血清miR-195-5p水平显著降低，IL-17水平显著升高（均P<0.05）。与治疗前比较，治疗后MPP患儿血清miR-195-5p水平显著升高，血清IL-17水平显著降低（均P<0.05）。Pearson法分析显示，MPP患儿血清miR-195-5p水平与IL-17水平呈负相关（r=-0.413，P<0.05）。ROC曲线分析显示，血清miR-195-5p诊断MPP的ROC曲线下面积（area under the curve，AUC）为0.875，敏感度为82.0%，特异度为88.2%，截断值为3.869；IL-17诊断MPP的AUC为0.736，敏感度为66.0%，特异度为74.1%，截断值为152.919 ng·ml^-1；联合诊断MPP的AUC为0.903，敏感度为96.0%，特异度为97.1%。多因素Logistic回归分析表明，血清miR-195-5p、IL-17是MPP发生的影响因素。结论：MPP患儿血清miR-195-5p呈低表达，IL-17呈高表达，两者联合检测能明显提高MPP诊断准确率，更有利于临床用药指导。

Objective: To study the early changes of serum miR-195-5p and interleukin-17(IL-17) levels in children with Mycoplasma pneumonia(MPP) and their diagnostic value. Methods: A total of 150 children with MPP who visited our hospital's Pediatric Respiratory Clinic from February 2018 to February 2019 were selected as MPP group, and 50 children with healthy physical examination were selected as control group during the same period. Real-time fluorescent quantitative PCR(qRT-PCR) method was used to determine the expression of miR-195-5p in the serum of children in each group. Enzyme-linked immunosorbent assay(ELISA) was used to detect the level of serum IL-17 of children in the two groups. The levels of serum miR-195-5p and IL-17 in each group were compared. Pearson method was used to analyze the correlation between serum miR-195-5p and IL-17 levels. Receiver operating characteristic (ROC) curve was drawn to evaluate serum miR-195-5p and IL-17 levels and the early predictive value of the combined detection of the two for MPP. Logistic regression was used to analyze the influencing factors of MPP. Results: Compared with the control group, the serum miR-195-5p level of children in the MPP group was significantly decreased, and the serum IL-17 level was significantly increased in the MPP group(both P<0.05). Compared with before the treatment, the level of serum miR-195-5p in children with MPP increased significantly and the level of serum IL-17 decreased significantly after treatment(both P<0.05). Pearson analysis showed that serum miR-195-5p and IL-17 levels in children with MPP were negatively correlated(P<0.05). ROC curve analysis results showed that the area under the curve(AUC) of serum miR-195-5p for diagnosis of MPP was 0.875, with the sensitivity being 82.0%, the specificity being 88.2%, and the cut-off value being 3.869. The AUC of IL-17 for diagnosis of MPP was 0.736, with the sensitivity being 66.0%, the specificity being 74.1%, and the cut-off value being 152.919 ng·ml^-1. The AUC of the combined diagnosis of MPP was 0.903, with the sensitivity being 96.0%, and the specificity being 97.1%. Multivariate Logistic regression analysis showed that serum miR-195-5p and IL-17 were the influencing factors of MPP. Conclusion: Serum miR-195-5p expression is low in children with MPP, and IL-17 expression is high. The combined detection of the two can significantly improve the diagnostic accuracy of MPP, which is more conducive to clinical medication guidance.

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