miRNA-194和miRNA-192在肝癌患者中的表达及临床意义-现代医学

miRNA-194和miRNA-192在肝癌患者中的表达及临床意义

单位：1. 自贡市精神卫生中心老年内科, 四川自贡 643020;
2. 自贡市精神卫生中心肿瘤科, 四川自贡 643020

分类号：R735.7

出版年·卷·期（页码）：2022·50·第六期（748-755）

摘要：

目的：探究肝细胞癌(HCC)患者癌组织中miRNA-194、miRNA-192的表达及其临床意义。方法：选取2016年3月至2017年3月自贡市精神卫生中心收治的67例肝癌患者，取手术后切除的癌组织以及其相应的癌旁正常组织。采用实时荧光定量PCR (qRT-PCR)法测定HCC组织及癌旁正常组织中miRNA-194、miRNA-192的表达情况，分析miRNA-194、miRNA-192与患者的性别、年龄、肿瘤分化程度、淋巴结转移、肿块大小等临床病理参数的关系；对所有HCC患者进行3年随访，采用Kaplan-Meier法分析miRNA-194、miRNA-192表达与HCC患者预后的关系；采用Cox回归分析影响HCC患者预后不良的危险因素。结果：HCC组织中miRNA-194、miRNA-192的表达水平显著低于癌旁组织(P＜0.05)；miRNA-194、miRNA-192水平与HCC患者性别、年龄、饮酒史、肝硬化、肿瘤数量无关(均P＞0.05)，与AJCC分期、甲胎蛋白、肿瘤大小、TNM分期、分化程度、淋巴结转移、静脉侵犯、脉管癌栓、CLIP评分有关(均P＜0.05)；Kaplan-Meier结果显示miRNA-194、miRNA-192低表达组生存率均低于高表达组，差异均有统计学意义(均P＜0.05)。多因素分析结果显示，miRNA-194、miRNA-192低表达是影响HCC患者预后不良的危险因素(P<0.05)。结论：miRNA-194、miRNA-192低表达可能与HCC的发生发展及不良预后有关，有望成为HCC潜在的研究靶点。

Objective: To explore the expression of miRNA-194 and miRNA-192 in hepatocellular carcinoma(HCC) patients and their clinical significance. Methods: A total of 67 HCC patients who were admitted to Zigong Mental Health Center from March 2016 to March 2017 were selected, and the cancer tissues resected through surgery and their corresponding normal tissues adjacent to the cancer were taken too. Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR) was used to determine the expression of miRNA-194 and miRNA-192 in HCC and adjacent tissues, the correlation between miRNA-194 and miRNA-192 and the patient's gender, age, tumor differentiation, lymph node metastasis, tumor size and other clinicopathological parameters was analyzed; all HCC patients were followed up for 3 years, Kaplan-Meier method was used to analyze the relationship between the expression of miRNA-194 and miRNA-192 and the prognosis of HCC patients; and COX regression analysis was used to analyze the risk factors affecting the poor prognosis of HCC patients. Results: The expression levels of miRNA-194 and miRNA-192 in HCC cancer tissues were significantly lower than those in adjacent tissues(P<0.05); the levels of miRNA-194 and miRNA-192 were not related to the gender, age, drinking history, liver cirrhosis, and the number of tumors in HCC patients(all P>0.05), but related to AJCC staging, alpha fetoprotein(AFP), tumor size, TNM staging, degree of differentiation, lymph node metastasis, venous invasion, vascular tumor thrombus, and CLIP score(all P<0.05); Kaplan-Meier results showed that the survival rates of miRNA-194 and miRNA-192 in low expression group were lower than those of high expression group,All differences showed statistical significance(all P<0.05); multivariate COX analysis showed that low levels of miRNA-194 and miRNA-192 were risk factors affecting the poor prognosis of HCC patients(all P<0.05). Conclusion: The low expression of miRNA-194 and miRNA-192 may be related to the occurrence, development and poor prognosis of HCC, and they are expected to become potential research targets for HCC.

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