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pH响应的二茂铁基纳米粒抑制下咽癌的研究
作者:林必成1  韩荣荣3  孙硕1 2  郭益玮1 2  赵立民2 
单位:1. 潍坊医学院 临床医学院, 山东 潍坊 261053;
2. 潍坊医学院附属医院 耳鼻咽喉科, 山东 潍坊 261035;
3. 潍坊市人民医院 耳鼻咽喉科, 山东 潍坊 261041
关键词:下咽癌 二茂铁基载药纳米粒 阿霉素 pH响应 活性氧 
分类号:R739.6
出版年·卷·期(页码):2022·50·第五期(535-542)
摘要:

目的:制备负载阿霉素(DOX)的二茂铁基载药纳米粒(APTES-FB-DOX),观察APTES-FB-DOX纳米粒理化特性及对人下咽鳞状细胞癌FaDu细胞的抑制作用。方法:采用沉淀聚合法制备二茂铁基聚合物(APTES-FB),通过搅拌离心使聚合物负载DOX得到APTES-FB-DOX纳米粒,应用MTT法、4,6-联脒-2苯基吲哚(DAPI)染色方法及活性氧(ROS)探针DCFH-DA观察纳米粒对细胞的作用。结果:制备的APTES-FB-DOX具有良好的理化性能。与DOX组相比,APTES-FB-DOX共培养组的L02细胞存活率明显较高(P<0.05),而对于FaDu细胞,APTES-FB-DOX共培养组的细胞存活率明显较低(P<0.01)。DOX摄取实验结果显示,与L929细胞相比,FaDu细胞明显观察到DOX的摄取。ROS实验表明,APTES-FB-DOX组产生的荧光明显强于DOX组。结论:制备的APTES-FB-DOX纳米粒较单一阿霉素对FaDu细胞的抑制作用更强,同时能减少DOX对外周组织细胞的毒性作用。

Objective:To prepare ferrocenyl nanoparticles(APTES-FB-DOX) loaded with doxorubicin(DOX), and observe its physical and chemical properties as well as its inhibitory effect on human hypopharyngeal squamous cell carcinoma FaDu cells. Methods:The precipitation polymerization was adopted to prepare ferrocenyl polymer(APTES-FB), and then the polymer was loaded with doxorubicin via stirring and centrifugation to obtain APTES-FB-DOX nanoparticles. The MTT assay, 4,6-amidine-2-phenylindole(DAPI) staining method and reactive oxygen species(ROS) probe DCFH-DA were used to observe the effect of APTES-FB-DOX on cells. Results:The prepared APTES-FB-DOX had good physical and medical properties. Compared with that in DOX group, the survival rate of L02 cells in APTES-FB-DOX group was significantly higher(P<0.05), while the survival rate of FaDu cells in APTES-FB-DOX group was significantly lower(P<0.01). The results of doxorubicin uptake experiment showed compared with L929 cells, doxorubicin uptake was more obviously observed in FaDu cells. The ROS experiment indicated that fluorescence produced by APTES-FB-DOX group was significantly stronger than that of DOX group. Conclusion:The prepared APTES-FB-DOX nanoparticles have stronger inhibitory effect on FaDu cells than doxorubicin alone, which could reduce the toxicity of doxorubicin to peripheral tissue cells at the same time.

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