目的：研究长链非编码RNA(lncRNA) INK4基因座的反义RNA(ANRIL)对呼吸道合胞病毒(RSV)诱导的气道上皮细胞紧密连接蛋白表达和炎症因子分泌的影响。方法：气道上皮细胞(9HTE)分成对照组(Control组)、RSV组(RSV诱导)、sh-NC组(转染shRNA control，RSV诱导)、sh-ANRIL组(转染ANRIL shRNA，RSV诱导)、sh-ANRIL+PMA组(转染ANRIL shRNA，NF-κB信号激活剂处理，RSV诱导)。酶联免疫吸附法(ELISA)分析培养液上清中白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平，蛋白质印迹法(Western blotting)分析MUC5AC、E-cadherin、occludin、p65蛋白表达。结果：与Control组比较，RSV组气道上皮细胞培养液上清中IL-6、IL-8、IL-1β、TNF-α水平升高，MUC5AC、p65蛋白表达水平升高，E-cadherin、occludin蛋白表达水平降低。与sh-NC组比较，sh-ANRIL组气道上皮细胞培养液上清中IL-6、IL-8、IL-1β、TNF-α水平降低，MUC5AC、p65蛋白表达水平降低，E-cadherin、occludin蛋白表达水平升高。与sh-ANRIL组比较，sh-ANRIL+PMA组气道上皮细胞培养液上清中IL-6、IL-8、IL-1β、TNF-α水平升高，MUC5AC、p65蛋白表达水平升高，E-cadherin、occludin蛋白表达水平降低。结论：下调ANRIL通过抑制NF-κB信号通路的激活提高RSV诱导的气道上皮细胞紧密连接蛋白表达，减少细胞分泌炎症因子。

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