lncRNA HAND2-AS1和GLUT-1在口腔鳞癌中的表达及其意义-现代医学

lncRNA HAND2-AS1和GLUT-1在口腔鳞癌中的表达及其意义

单位：南京市口腔医院口腔颌面外科, 江苏南京 210000

关键词：长链非编码RNA HAND2-AS1 葡萄糖转运蛋白-1 口腔鳞癌表达临床意义

分类号：R739.8

出版年·卷·期（页码）：2022·50·第四期（440-446）

摘要：

目的：检测口腔鳞癌患者中长链非编码RNA HAND2-AS1(lncRNA HAND2-AS1)和葡萄糖转运蛋白-1(GLUT-1)的表达情况，并探究两者与患者病理特征及预后的关系。方法：选取本院口腔科经病理检查确诊为口腔鳞癌患者68例，取其手术切除的口腔鳞癌组织和癌旁正常组织，采用实时荧光定量PCR法(qRT-PCR)分别检测癌组织及癌旁正常组织lncRNA HAND2-AS1与GLUT-1 mRNA表达水平，免疫组织化学法检测GLUT-1蛋白表达情况；通过Kaplan-Meier法绘制生存曲线评估lncRNA HAND2-AS1、GLUT-1蛋白表达对口腔鳞癌患者预后的影响；采用Cox回归分析口腔鳞癌患者预后的影响因素。结果：口腔鳞癌组织lncRNA HAND2-AS1表达水平低于癌旁正常组织，GLUT-1 mRNA表达水平、GLUT-1蛋白阳性表达率高于癌旁正常组织，差异有统计学意义(P＜0.05)。lncRNA HAND2-AS1低表达组术后5年总生存率低于高表达组，GLUT-1蛋白阳性组术后5年总生存率低于阴性组，差异有统计学意义(P＜0.05)。lncRNA HAND2-AS1、GLUT-1蛋白是影响口腔鳞癌患者预后的独立危险因素。结论：lncRNA HAND2-AS1在口腔鳞癌患者癌组织中的表达水平明显降低，GLUT-1 mRNA表达水平及GLUT-1蛋白阳性表达率明显升高，两者与患者临床病理特征及预后密切相关。

Objective:To detect the expression of long non-coding RNA HAND2-AS1(lncRNA HAND2-AS1) and glucose transporter 1(GLUT-1) in patients with oral squamous cell carcinoma, and to explore therelationship with pathological characteristics and prognosis of patients. Methods: A total of 68 patients with oral squamous cell carcinoma confirmed by pathological examination in the department of stomatology of our hospital were selected. The oral squamous cell carcinoma tissues and adjacent normal tissues resected during the surgery were taken. The expression level of lncRNA HAND2-AS1 and GLUT-1 mRNA in adjacent tissues and cancer tissues were detected with real-time fluorescent quantitative PCR(qRT-PCR), and the expression of GLUT-1 protein was detected with immunohistochemical method. The influence of lncRNA HAND2-AS1 and GLUT-1 protein expression on the prognosis of patients with oral squamous cell carcinoma was evaluated by drawing a survival curve with Kaplan-Meier method; and Cox regression analysis was used to analyze the prognostic factors of patients with oral squamous cell carcinoma. Results: The expression level of lncRNA HAND2-AS1 in oral squamous cell carcinoma group was lower than that in adjacent normal group, and the expression level of GLUT-1 mRNA and the positive expression rate of GLUT-1 protein were higher than those in the adjacent normal group, the difference was statistically significant(P<0.05). The 5-year overall survival rate of lncRNA HAND2-AS1 low expression group was lower than that of high expression group, and the 5-year overall survival rate of GLUT-1 protein positive group was lower than that of negative group, the difference was statistically significant(P<0.05). lncRNA HAND2-AS1 and GLUT-1 protein were independent risk factors affecting the prognosis of patients with oral squamous cell carcinoma. Conclusion: The expression level of lncRNA HAND2-AS1 in patients with oral squamous cell carcinoma is significantly decreased, and the expression level of GLUT-1 mRNA and the positive expression rate of GLUT-1 protein is significantly increased, they are closely related to the clinicopathological characteristics and prognosis of patients.

参考文献：

[1] 麦明思,赵焱,马锴.口腔鳞癌miR-762的表达及其与癌细胞增殖和转移的关系[J].现代医学,2021,49(1):18-23.
[2] FENG X,LUO Q,WANG H,et al.MicroRNA-22 suppresses cell proliferation,migration and invasion in oral squamous cell carcinoma by targeting NLRP3[J].J Cell Physiol,2018,233(9):6705-6713.
[3] CHEN J,LIN Y,JIA Y,et al.LncRNA HAND2-AS1 exerts anti-oncogenic effects on ovarian cancer via restoration of BCL2L11 as a sponge of microRNA-340-5p[J].J Cell Physiol,2019,234(12):23421-23436.
[4] 周立强,李世豪,刘力,等.重组甲硫氨酸酶调控PI3K/Akt/Glut-1通路抑制有氧糖酵解促进胃癌细胞凋亡[J].南方医科大学学报,2020,40(1):27-33.
[5] BRANDS R C,KÖHLER O,RAUTHE S,et al.The prognostic value of GLUT-1 staining in the detection of malignant transformation in oral mucosa[J].Clin Oral Investig,2017,21(5):1631-1637.
[6] 中华口腔医学会口腔病理学专业委员会.口腔癌及口咽癌病理诊断规范[J].中国口腔颌面外科杂志,2020,18(4):289-296.
[7] RAMOS-VARA J A.Principles and methods of immunohistochemistry[J].Methods Mol Biol,2017,1641(1):115-128.
[8] 田胜伟,张光远,许斌,等.LINC00958与肿瘤预后的Meta分析及生物信息学分析[J].东南大学学报(医学版),2021,40(1):27-33.
[9] 兰飞,赵鹏,刘冬,等.LncRNA DANCR调控非小细胞肺癌细胞的凋亡和上皮间质转化[J].现代医学,2020,48(1):75-82.
[10] 张鹏,赵军伟,安红丽,等.肺腺癌转移相关转录因子1在口腔鳞状细胞癌组织中的表达及临床意义[J].局解手术学杂志,2019,28(9):715-718.
[11] GONG J,FAN H,DENG J,et al.LncRNA HAND2-AS1 represses cervical cancer progression by interaction with transcription factor E2F4 at the promoter of C16orf74[J].J Cell Mol Med,2020,24(11):6015-6027.
[12] YAN Y,LI S,WANG S,et al.Long noncoding RNA HAND2-AS1 inhibits cancer cell proliferation,migration,and invasion in esophagus squamous cell carcinoma by regulating microRNA-21[J].J Cell Biochem,2019,120(6):9564-9571.
[13] 刘德裕,翦新春,徐普,等.长链非编码RNA HAND2-AS1对口腔鳞状细胞癌增殖和糖代谢的影响[J].实用医学杂志,2020,36(13):1739-1744.
[14] ZAMBRANO A,MOLT M,URIBE E,et al.Glut 1 in cancer cells and the inhibitory action of resveratrol as a potential therapeutic strategy[J].Int J Mol Sci,2019,20(13):3374-3379.
[15] QAMAR S,FATIMA S,REHMAN A,et al.Glucose transporter 1 overexpression in oral squamous cell carcinoma[J].J Coll Physicians Surg Pak,2019,29(8):724-727.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录