Objective:To explore the effect of circ_0000337 on the proliferation and apoptosis of glioma cells and its regulatory effect on miR-1299.Methods:The qRT-PCR method was used to detect the expression of circ_0000337 and miR-1299 in normal brain tissue and glioma tissue.In vitro cultured human glioma cells U251,si-NC (si-NC group),si-circ_0000337(si-circ_0000337 group),miR-NC (miR-NC group),miR-1299 mimics (miR-1299 group),si-circ_0000337 and anti-miR-NC (si-circ_0000337+anti-miR-NC group),si-circ_0000337 and anti-miR-1299(si-circ_0000337+anti-miR-1299 group) were transfected into U251 cells.CCK-8 experiment and flow cytometry were used to detect cell proliferation and apoptosis,respectively.The dual luciferase reporter gene experiment was used to detect the targeting relationship between circ_0000337 and miR-1299.Results:Compared with normal brain tissue,the expression level of circ_0000337 in glioma tissue was increased (P<0.05),while the expression level of miR-1299 was decreased (P<0.05).Compared with the si-NC group,the cell viability in the si-circ_0000337 group was decreased (P<0.05),while the apoptosis rate was increased (P<0.05).Compared with the miR-NC group,the cell viability of the miR-1299 group was decreased (P<0.05),while the apoptosis rate was increased (P<0.05).circ_0000337 could target miR-1299.Compared with the si-circ_0000337+anti-miR-NC group,the cell viability in the si-circ_0000337+anti-miR-1299 group was increased (P<0.05),while the apoptosis rate was decreased (P<0.05).Conclusion:Inhibiting the expression of circ_0000337 could inhibit the proliferation of glioma cells and promote cell apoptosis by targeting the expression of miR-1299. |
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