Objective:To explore the effect of circ_0004771 on the proliferation,migration and invasion of glioma U251 cells and its molecular mechanism.Methods:33 cases of glioma tissue specimens and normal brain tissues were selected,and the expression levels of circ_0004771 and miR-924 were detected by real-time fluorescent quantitative PCR (RT-qPCR).The glioma cell line U251 was randomly divided into small interfering RNA negative sequenc (si-NC) group (the cells were transfected with si-NC),circ_0004771 small interfering RNA (si-circ_0004771) group (the cells were transfected with si-circ_0004771),mimic control sequence (miR-NC) group (the cells were transfected with miR-NC),miR-924 mimic (miR-924) group (the cells were transfected with miR-924),si-circ_0004771+inhibitor negative sequence (anti-miR-NC) group (the cells were co-transfected with si-circ_0004771 and anti-miR-NC),si-circ_0004771+miR-924 inhibitor (anti-miR-924) group (the cells were co-transfected with si-circ_0004771 and anti-miR-924).And then cell viability was detected by cell counting kit 8(CCK-8).The number of cell clone formation was detected by clone formation experiment.The cell scratch healing rate was detected by scratch experiment.The number of cell invasion was detected by Transwell chamber.The protein expression of E-cadherin and N-cadherin were detected by Western blot method.The targeting relationship between circ_0004771 and miR-924 was detected by the dual luciferase reporter experiment.Results:Compared with normal brain tissue,the expression level of circ_0004771 in glioma tissue was increased,and the expression of miR-924 was decreased (P<0.05).Compared with the si-NC group (or miR-NC group),the activity of glioma U251 cells,clone formation number,scratch healing rate,invasion number and the expression of N-cadherin protein in si-circ_0004771 group (or miR-924 group) were decreased (P<0.05),but the expression of E-cadherin protein was increased (P<0.05).circ_0004771 targeted and regulated the expression of miR-924.Compared with the si-circ_0004771 and anti-miR-NC groups,the activity of glioma U251 cells,clone formation number,scratch healing rate,invasion number and the expression of N-cadherin protein in si-circ_0004771+anti-miR-924 group were increased (P<0.05),but the expression of E-cadherin protein was decreased (P<0.05).Conclusion:Down-regulating the expression of circ_0004771 may inhibit the proliferation,migration and invasion of glioma U251 cells by targeting up-regulation of miR-924. |
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