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一氧化碳释放分子对呼吸心跳骤停大鼠海马组织及ATP、LA表达的影响
作者:廖涛  刘超 
单位:陕西省商洛市中心医院 急诊医学科, 陕西 商洛 726000
关键词:CO释放分子 呼吸心跳骤停 复苏效果 海马组织 三磷酸腺苷 乳酸 
分类号:R541.78
出版年·卷·期(页码):2022·50·第一期(23-29)
摘要:

目的:探究一氧化碳释放分子(CORM)对呼吸心跳骤停(CA)大鼠海马组织及三磷酸腺苷(ATP)、乳酸(LA)表达的影响。方法:将大鼠平均分为阴性对照组(NC组)、无差异对照组(NS组)和一氧化碳释放分析组(CORM组),制备心跳骤停大鼠模型,分别比较大鼠窒息后的相关指标和平均动脉压(MAP)水平;检测3组大鼠血清中神经元特异性烯醇化酶(NSE)水平和ATP、LA含量的变化;用TUNEL检测大鼠脑组织中海马细胞凋亡情况;用HE、尼氏染色观察大鼠脑组织病理学的改变,及观察大鼠脑组织超微结构的变化。结果:和NS组大鼠相比,CORM组大鼠出现自主心率时间明显减少(P<0.05),复苏成功率升高,但组间无明显差异(P>0.05);NS组和CORM组大鼠窒息至CA时间和心肺复苏后MAP值比较没有显著的变化(P>0.05)。NS组大鼠血清中NSE水平明显高于NC组(P<0.05),干预后的CORM组大鼠血清中NSE水平明显低于NS组(P<0.05)。和NC组相比,NS组大鼠脑组织中ATP的含量明显减少,LA的含量显著增多(P<0.01);干预后的CORM组和NS组大鼠相比,ATP含量明显增高,LA含量得到了明显的抑制(P<0.01)。TUNEL、HE和尼氏染色结果发现,NS组大鼠海马神经细胞大量凋亡,且细胞出现部分变性,排列紊乱,胞浆水肿明显,细胞周围间隙增宽,海马神经细胞尼氏体减少,NS组大鼠海马神经细胞各细胞器结构破坏,线粒体肿胀,部分出现空泡,嵴排列不规则;和NS组大鼠相比,CORM组大鼠海马神经细胞凋亡得到抑制,且海马组织得到明显改善。结论:CO释放分子可改善心跳骤停大鼠复苏效果和海马组织病理学损伤程度,同时能增加能量代谢中ATP的含量,减少LA的含量,进而保护脑组织。

Objective: To explore the effects of carbon monoxide releasing molecules on neuronal apoptosis and the expression of ATP and LA in rats with respiratory and cardiac arrest. Methods: The rats were equally divided into NC group, NS group and CORM group to prepare cardiac arrest rat models, and compared the relevant indexes and MAP levels after asphyxia in rats; detect the levels of NSE and ATP and LA in the serum of the three groups of rats use TUNEL to detect hippocampal cell apoptosis in rat brain tissue;use HE and Nissl stainingto observe the pathological changes of rat brain tissue, andobserve the changes of rat brain tissue ultrastructure. Results: Compared withNS group, rats in the CORM group showed a significant reduction in the time of autonomous heart rate(P<0.05), and the success rate of resuscitation increased, but there was no significant difference between the two groups(P>0.05). There was no significant change inthe time from asphyxiation to CA and the MAP value after cardiopulmonary resuscitation between the NS group and the CORM group(P>0.05). The level of NSE in the serum of rats in the NS group was significantly higher than that in the NC group(P<0.05), and the level of NSE in the serum of rats in the CORM group after intervention was significantly lower than that in the NS group(P<0.05). Compared with the NC group, the ATP content in the brain tissue of the NS group was significantly reduced, and the LA content was significantly increased(P<0.01); afterintervention, the CORM group was significantly higher than the NS group. The content was significantly suppressed(P<0.01). The results of TUNEL, HE and Nissl staining showed that a large number ofhippocampal nerve cells in the NS group were apoptotic, and the cells showed partial degeneration, disordered arrangement, obvious cytoplasmic edema, widening of the intercellular space, and decreased Nissl body of hippocampal nerve cells in the NS group. The organelles of hippocampal nerve cells of rats in the group were damaged, mitocho.Conclusion:CO releasing molecules can improve the resuscitation effect of cardiac arrest rats and the degree of hippocampal histopathological damage, and at the same time can increase the content of ATP in energy metabolism, reduce the content of LA, thus protect the brain tissue.

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