Objective: To explore the effects of carbon monoxide releasing molecules on neuronal apoptosis and the expression of ATP and LA in rats with respiratory and cardiac arrest. Methods: The rats were equally divided into NC group, NS group and CORM group to prepare cardiac arrest rat models, and compared the relevant indexes and MAP levels after asphyxia in rats; detect the levels of NSE and ATP and LA in the serum of the three groups of rats use TUNEL to detect hippocampal cell apoptosis in rat brain tissue;use HE and Nissl stainingto observe the pathological changes of rat brain tissue, andobserve the changes of rat brain tissue ultrastructure. Results: Compared withNS group, rats in the CORM group showed a significant reduction in the time of autonomous heart rate(P<0.05), and the success rate of resuscitation increased, but there was no significant difference between the two groups(P>0.05). There was no significant change inthe time from asphyxiation to CA and the MAP value after cardiopulmonary resuscitation between the NS group and the CORM group(P>0.05). The level of NSE in the serum of rats in the NS group was significantly higher than that in the NC group(P<0.05), and the level of NSE in the serum of rats in the CORM group after intervention was significantly lower than that in the NS group(P<0.05). Compared with the NC group, the ATP content in the brain tissue of the NS group was significantly reduced, and the LA content was significantly increased(P<0.01); afterintervention, the CORM group was significantly higher than the NS group. The content was significantly suppressed(P<0.01). The results of TUNEL, HE and Nissl staining showed that a large number ofhippocampal nerve cells in the NS group were apoptotic, and the cells showed partial degeneration, disordered arrangement, obvious cytoplasmic edema, widening of the intercellular space, and decreased Nissl body of hippocampal nerve cells in the NS group. The organelles of hippocampal nerve cells of rats in the group were damaged, mitocho.Conclusion:CO releasing molecules can improve the resuscitation effect of cardiac arrest rats and the degree of hippocampal histopathological damage, and at the same time can increase the content of ATP in energy metabolism, reduce the content of LA, thus protect the brain tissue. |