过表达LncRNA SNHG7通过miR-342-3p/FOXM1轴促进胃癌细胞增殖、侵袭及其EMT-现代医学

过表达LncRNA SNHG7通过miR-342-3p/FOXM1轴促进胃癌细胞增殖、侵袭及其EMT

单位：1. 湖南省肿瘤医院结直肠外科, 湖南长沙 410013;
2. 湖南省肿瘤医院肝胆外科, 湖南长沙 410013

关键词：Lnc RNA SNHG7 miR-342-3p FOXM1 胃癌增殖 EMT

分类号：R735.2

出版年·卷·期（页码）：2022·50·第一期（1-9）

摘要：

目的：探讨lncRNA SNHG7 在胃癌MGC-803细胞中的表达及其对MGC-803细胞增殖、侵袭及上皮间质转化(epithelial-mesenchymal transition, EMT)的影响。方法：收集胃癌组织及其癌旁正常组织标本，培养胃癌细胞(MGC-803)和癌旁正常组织细胞(GES-1)，采用Real-time PCR检测SNHG7、miR-342-3p、FOXM1在胃癌组织、癌旁正常组织、MGC-803细胞和GES-1细胞中的表达。通过MTT法、细胞侵袭和Western blot实验检测下调lncRNA SNHG7对MGC-803细胞增殖、侵袭及EMT的影响。通过miRcode软件和双荧光素酶报告基因活性检测方法剖析lncRNA SNHG7和miR-342-3p之间的关系；下调miR-342-3p，分析MGC-803细胞的发展趋势。同时上调lncRNA SNHG7和miR-342-3p，检测lncRNA SNHG7通过miR-342-3p对MGC-803细胞的增殖、侵袭及EMT的影响；TargetScan软件分析miR-342-3p与FOXM1的结合位点，双荧光素酶报告检测miR-342-3p与FOXM1之间的靶向关系。MTT法检测下调FOXM1对MGC-803细胞增殖的影响。细胞侵袭和Western blot分别检测下调FOXM1对MGC-803细胞侵袭和EMT的影响。结果：与GES-1细胞相比，MGC-80细胞中lncRNA SNHG7高表达(P<0.01)，miR-342-3p低表达(P<0.01)，FOXM1高表达(P<0.01)。与癌旁正常组织相比，胃癌组织中lncRNA SNHG7高表达(P<0.01)，miR-342-3p低表达(P<0.01)，FOXM1高表达(P<0.01)。下调lncRNA SNHG7抑制MGC-803细胞的增殖、侵袭及EMT。LncRNA SNHG7与miR-342-3p之间具有负调控及靶向关系。下调miR-342-3p促进MGC-803细胞的增殖、侵袭及EMT；上调lncRNA SNHG7通过miR-342-3p促进MGC-803细胞的增殖、侵袭及EMT。miR-342-3p与FOXM1之间具有负调控及靶向关系。下调FOXM1对MGC-803细胞的增殖、侵袭及EMT具有抑制作用。lncRNA SNHG7通过miR-342-3p上调FOXM1。结论：过表达LncRNA SNHG7通过miR-342-3p/FOXM1轴促进胃癌细胞增殖、侵袭及其EMT。

Objective:To investigate the expression of lncRNA SNHG7 in gastric cancer MGC-803 cells and its effects on the proliferation, invasion and EMT of MGC-803 cells.Methods:Specimens of gastric cancer tissues and adjacent normal tissues were collected，gastric cancer cells(MGC-803) and adjacent normal tissue cells(GES-1) were cultured，real-time PCR was used to detect the expression of SNHG7, miR-342-3p and FOXM1 in gastric cancer tissues, adjacent normal tissues, MGC-803 cells and GES-1 cells.The effects of down-regulation of lncRNA SNHG7 on proliferation, invasion and EMT of MGC-803 cells were detected by MTT assay, cell invasion and Western blot assay.The relationship between lncRNA SNHG7 and miR-342-3p was analyzed using miRcode software and dual luciferase reporter gene activity detection method. MiR-342-3p was down-regulated to analyze the development trend of MGC-803 cells, meanwhile, lncRNA SNHG7 and miR-342-3p were up-regulated，the effects of lncRNA SNHG7 on the proliferation, invasion and EMT of MGC-803 cells via miR-342-3p were detected. TargetScan software analyzed the binding sites between miR-342-3p and FOXM1，dual luciferase reporting detected the targeting relationship between miR-342-3p and FOXM1. MTT assay was used to detect the effect of down-regulated FOXM1 on MGC-803 cell proliferation. The effects of down-regulated FOXM1 on MGC-803 cell invasion and EMT were detected by cell invasion and Western blot respectively.Results:Compared with GES-1 cells, lncRNA SNHG7 was highly expressed in MGC-80 cells(P<0.01), miR-342-3p was lowly expressed(P<0.01), and FOXM1 was highly expressed(P<0.01)。Compared with normal tissues adjacent to cancer, lncRNA SNHG7 was highly expressed in gastric cancer tissues(P<0.01), miR-342-3p was lowly expressed(P<0.01), and FOXM1 was highly expressed(P<0.01). Downregulation of lncRNA SNHG7 inhibited proliferation, invasion and EMT of MGC-803 cells. miR-342-3p had a negative regulation and targeting relationship with FOXM1. Downregulation of miR-342-3p promoted the proliferation, invasion and EMT of MGC-803 cells. The up-regulation of lncRNA SNHG7 promoted the proliferation, invasion and EMT of MGC-803 cells through miR-342-3. MiR-342-3p had a negative regulation and targeting relationship with FOXM1. The down-regulation of FOXM1 could inhibit the proliferation, invasion and EMT of MGC-803 cells；lncRNA SNHG7 up-regulated FOXM1 by miR-342-3p.Conclusion:Overexpression of lncRNA SNHG7 promoted the proliferation, invasion and EMT of gastric cancer cells through miR-342-3p/FOXM1 axis.

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