PM2.5长期暴露诱导人肺支气管上皮细胞恶性转化的研究-现代医学

PM2.5长期暴露诱导人肺支气管上皮细胞恶性转化的研究

单位：1. 东南大学公共卫生学院, 江苏南京 210009;
2. 南京医科大学附属儿童医院质量管理办公室, 江苏南京 210009;
3. 东南大学附属中大医院风湿免疫科, 江苏南京 210009

分类号：R122

出版年·卷·期（页码）：2021·49·第十一期（1303-1306）

摘要：

目的：研究PM2.5长期暴露诱导人肺支气管上皮细胞（16HBE细胞）恶性转化的能力。方法：使用100μg·ml^-1的PM2.5连续长期暴露16HBE细胞，并设立对照组。采用CCK-8法测定细胞增殖能力，EdU法检测细胞增殖活性，Transwell法评价细胞迁移与侵袭能力，集落形成实验检测细胞克隆形成能力。结果：CCK-8结果显示PM2.5长期暴露后的16HBE细胞增殖能力较对照组显著增强（P<0.01），随着暴露代数增加，细胞增殖能力逐渐升高；EdU结果提示PM2.5长期暴露后细胞的活性显著提升（P<0.001）；与对照组相比，PM2.5暴露可显著增强16HBE细胞的迁移、侵袭及克隆形成能力（P<0.01）。结论：经PM2.5长期诱导的16HBE细胞可逐渐恶性转化。

Objective: To study the malignant transformation ability of human pulmonary bronchial epithelial cells (16HBE cells)induced by fine particulate matter (PM2.5) long-term exposure.Methods: 16HBE cells were continuously exposed to 100 μg·ml^-1 PM2.5 for a long time, and control group was set up. Cell proliferation was measured by CCK-8 method, cell proliferation activity was measured by EdU method, cell migration and invasion ability were evaluated by Transwell method, and cell clonal formation ability was detected by clonal formation assay.Results:CCK-8 showed that the proliferation of 16HBE cells increased significantly after PM2.5 long-term exposure compared with the control group (P<0.01), and the proliferation of 16HBE cells in treatment group increased gradually with the increase of passage number. EdU results showed that the activity of cells in treatment group increased significantly after long-term exposure of PM2.5 (P<0.001). Compared with the control group, PM2.5 exposure significantly enhanced the migration, invasion and clonal formation of 16HBE cells (P<0.01). Conclusions:16HBE cells induced by PM2.5 for a long time can be transformed into malignant cells gradually.

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