Objective: To investigate the effects of microRNA-448 (miR-448) on the proliferation and apoptosis of synovial cells in rheumatoid arthritis and its mechanism. Methods: Rheumatoid arthritis synovial cells MH7A were divided into Control group, Anti-NC group (transfected with inhibitor control), Anti-miR-448 group (transfected with miR-448 inhibitor), Anti-miR-448+si-NC group (co-transfected with miR-448 inhibitor, siRNA control), Anti-miR-448+si-PDCD5 group (co-transfected with miR-448 inhibitor, PDCD5 siRNA). Cell proliferation was detected by CCK-8, cell cycle was detected by PI single staining, cell apoptosis was detected by Annexin V-FITC/PI double staining, and protein expressions of C-Caspase-3, cyclin D1 and p21 were detected by Western blot. The luciferase reporting system was used to identify the targeting relationship between miR-448 and PDCD5. Results: Compared with Control group and Anti-NC group, Anti-miR-448 group cell survival rate decreased[(100.00±9.52)%,(99.71±10.36)% vs (52.62±4.47)%，P<0.05], apoptosis rate increased[(4.32±0.35)%,(4.18±0.62)% vs (12.51±1.32)%，P<0.05], G0/G1 phase ratio increased[(48.51±3.26)%,(50.80±6.20)% vs (63.81±5.41)%，P<0.05], and C-Caspase-3 and p21 protein increased, and the expression level of cyclin D1 protein decreased. Compared with the Anti-miR-448+si-NC group, the survival rate of rheumatoid arthritis synovial cells in the Anti-miR-448+si-PDCD5 group was increased [(100.00±10.35)% vs (147.84±16.50)%, P<0.05], cell G0/G1 phase ratio decreased [(64.08±6.10)% vs (53.11±4.75)%, P<0.05], cell apoptosis rate decreased [(13.05±1.16)% vs (6.92±0.35)%, P<0.05], the expression levels of C-Caspase-3, p21 and PDCD5 protein decreased, and the expression level of cyclin D1 protein increased. Down-regulation of miR-448 targeted up-regulation of PDCD5 expression. Conclusion: Down-regulation of miR-448 inhibits the proliferation of rheumatoid arthritis synovial cells, blocks the cell cycle and induces apoptosis by targeting the up-regulation of PDCD5 expression. |
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