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原发性肝癌患者外周血PD-1与IFN-γ的表达及临床意义
作者:胡金伟  张晓进  桑国耀 
单位:新疆医科大学第一附属医院 医学检验中心, 新疆 乌鲁木齐 830054
关键词:外周血单个核细胞 程序性死亡受体-1 干扰素γ 原发性肝癌 
分类号:R735.7
出版年·卷·期(页码):2021·49·第七期(733-736)
摘要:

目的:研究外周血中程序性死亡受体-1(PD-1)和干扰素γ(IFN-γ)在原发性肝癌(PHC)患者中的表达水平,探讨其在PHC发病中的作用。方法:选取自2018年8月至2020年8月于我院接受治疗的62例PHC患者为PHC组,同期46例慢性乙型肝炎(CHB)患者为CHB组,另选30例健康体检者为对照组。采用流式细胞术检测外周血单个核细胞(PBMC)中CD4+T细胞和CD8+T细胞中PD-1的表达,同时采用酶联免疫吸附测定(ELISA)法检测血清中IFN-γ水平,并分析PHC患者体内PD-1与IFN-γ的相关性。结果:与对照组和CHB组相比,PHC患者外周血PD-1表达及血清中IFN-γ水平均显著升高(P<0.01),且CHB组高于对照组,差异均具有统计学意义(均P<0.05)。且PHC患者体内PD-1的表达与IFN-γ水平呈正相关(r=0.526,P<0.05)。结论:PHC患者外周血中PD-1和IFN-γ的表达水平均升高,且PD-1的表达与IFN-γ水平呈正相关。

Objective: To investigate the expression levels of programmed cell death-1(PD-1)and interferon γ(IFN-γ)in peripheral blood in patients with primary hepatic carcinoma (PHC), and explore their role in the pathogenesis of PHC.Methods: 62 patients with PHC who were diagnosed and treated in our hospital from August 2018 to August 2020 were selected asPHC group, 46 patients with chronic hepatitis B (CHB) during the same period as CHB group, another 30 healthy people were selected as control group. Flow cytometry was used to detect the expression of PD-1 in CD4+T and CD8+T cells in peripheral blood mononuclear cells (PBMC).The level of IFN-γ in serum was detected by enzyme linked immunosorbent assay(ELISA), and the correlation between PD-1 and IFN-γ was analyzed in PHC group. Results: The expression ofPD-1 and the level of IFN-γ in PHC group were significantly increased than those in control group and CHB group, moreover, CHB group was higher than control group, the differences were all statistically significant (P<0.05). And the expression of PD-1 was positively correlated with IFN-γ in PHC group (r=0.526, P<0.05).Conclusion: The expression level of PD-1 and the level of IFN-γ in peripheral blood of PHC patients are increased and positively correlate.

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