目的：利用正交试验设计法，探讨超声参数对膀胱癌T24细胞膜通透性的影响。方法：设定超声声强、微泡/细胞悬液体积比、占空比、辐照时间为正交试验设计的4个因素，每个因素分别设置4个水平，即超声声强设0.4、0.6、0.8、1.0 W·cm^-2，微泡/细胞悬液体积比设10%、20%、30%、40%，占空比设10%、20%、30%、40%，辐照时间设1、2、3、4 min，按照4因素4水平生成正交试验设计表，共16个实验组，每组按照正交试验设计表进行超声辐照，辐照后采用荧光显微镜检测FD40S染色阳性率。结果：不同因素对细胞膜通透性的影响为超声声强>微泡/细胞悬液体积>占空比>辐照时间；各因素不同水平的影响程度由大至小为：超声声强0.6、0.4、1.0、0.8 W·cm^-2，辐照时间1、2、3、4 min，占空比40%、10%、20%、30%，微泡/细胞悬液体积10%、20%、30%、40%。结论：超声参数对膀胱癌T24细胞膜通透性影响最优组合为：超声声强0.6W·cm^-2、辐照时间1 min、占空比40%、微泡/细胞悬液体积10%。

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