Objective: To study the association between ADAM33 gene polymorphism and TGF-β/ERK signalpathway in Uygur asthmatic children in Urumqi.Methods: 74 asthmatic Uighur children(3-14 years old)were selected as case group. At the same time, 48 healthy children of the same ethnic group were set as the control group. PCR polymerase chain reaction was used for V4 ADAM33 gene loci single nucleotide polymorphism. ELISA method was used to detect serum IgE, TGF-β1, ERK level. Finally, correlation analysis of V4 locus, TGF-1 and ERK in the case group was conducted.Results: (1) Three genotype frequency distribution in V4 locus was statistically significant difference between the case and the control group(χ2=6.322,P<0.05), the allele frequency difference between the two groups was statistically significant(χ2=6.039,P<0.05). (2) The serum IgE level of the case group was significantly higher than that of the control group(t=3.912,P<0.001). Serum TGF-β1 level in the case group was significantly increased, and the difference was statistically significant(t=7.106,P<0.001). Serum ERK level in the case group was significantly increased, and the difference was statistically significant (t=5.379,P<0.001).(3) Serum TGF-β1 level in the case group waspositively correlated with ERK level(r=0.439,P<0.01). The V4 locus was significantly positively correlated with ERK(r=0.238,P=0.041). The V4 site was not significantly correlated with TGF-β1(r=0.191,P=0.103).Conclusion: (1) ADAM33's V4 gene is the susceptibility gene of Uygur children with asthma;(2) ADAM33 gene polymorphism is associated with TGF-β/ERK signal transduction pathway in Uygur asthmatic children in Urumqi. |
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