Objective: To detect the key factor of tumor stem cell octamer binding transcription factor 4 (OCT4) mRNA and tissue protein expression in cervical cancer, and to quantitatively detect the plasma protein content of OCT4 in peripheral blood, and to explore its occurrence, development, infiltration and transfer in cervical cancer. Methods: Fluorescence quantitative RT-PCR, enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to quantitatively detect OCT4 mRNA, tissue protein and plasma protein content, and statistical ROC curve was used to further evaluate the accuracy, specificity and sensitivity of this indicator. Results: (1) The expression of OCT4 mRNA in cervical cancertissues (1.733±0.968) clearly exceeded the expression in CIN Ⅱ-Ⅲ tissues (1.697±1.431) andcervicitis tissues (0.636±0.374), and there were significant differences, P<0.05; (2) immunohistochemistry result showed that with the increase of malignant degree of cervical lesions, the expression rate of OCT4 protein increased, and the expression rate of OCT4 protein in cervical cancer tissue was 80% (32/40), which was significantly higher than the expression rate incervicitis tissue 31.57% (6/19), and P<0.05; (3) ELISA test results in peripheral blood showed that comparing cervicat cancer with CIN Ⅱ-Ⅲ or chronic cervicitis, the difference in plasma concentration of OCT4 was statistically significant (P<0.05), but the difference between CIN Ⅱ-Ⅲ and inflammation group was not statistically significant (P>0.05). (4) After drawing the ROC curve, the area under the curve (AUC) of the OCT4 gene was 0.895. Next, the author started from the OCT4 concentration and focused on the analysis of specificity and sensitivity, AUC of OCT4 plasma level was0.773. Obviously, the detection accuracy of OCT4 gene and plasma level was relatively high. Conclusion: The expression of tumor stem cell marker OCT4 is up-regulated, which provides reliable experimental data for the diagnosis of cervical cancer.The expression of this transcription factor in tissue and plasma is likely to become a prognostic biomarker or therapeutic target.
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