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let-7c调控非小细胞肺癌细胞生长和自噬的研究
作者:郑爽  许林晖  夏书月 
单位:沈阳医学院附属中心医院 呼吸与危重症医学科, 辽宁 沈阳 110000
关键词:微小RNA let-7c 细胞自噬 非小细胞肺癌 
分类号:R734.2
出版年·卷·期(页码):2021·49·第四期(389-394)
摘要:

目的:探讨let-7c在非小细胞肺癌细胞生长和自噬中的作用。方法:采用测序方法找寻肺癌组织中表达差异显著的miRNAs,通过RT-qPCR验证测序结果,采用细胞转染技术对非小细胞肺癌进行过表达构建,随后使用RT-qPCR检测转染效率,并经CCK8实验、Western blot实验及免疫荧光实验验证let-7c对肺癌细胞生长和自噬调控因子的影响。结果:let-7c在肺癌组织以及肺癌细胞中的表达降低,过表达let-7c不仅抑制非小细胞肺癌细胞增殖,同时还抑制非小细胞肺癌细胞自噬的发生。结论:let-7c在非小细胞肺癌中发挥抑癌基因作用,有望成为潜在的治疗靶点。

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