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口腔鳞癌miR-762的表达及其与癌细胞增殖和转移的关系
作者:麦明思  赵焱  马锴 
单位:三亚市人民医院 口腔科, 海南 三亚 572000
关键词:口腔鳞癌 微小RNA-762 增殖 凋亡 预后 
分类号:R739.85
出版年·卷·期(页码):2021·49·第一期(18-23)
摘要:

目的: 检测口腔鳞癌微小RNA-762(miR-762)的表达,探讨其与癌细胞增殖和转移的相关性。方法: 收集确诊为口腔鳞癌的患者共62例。肿瘤组织作为观察组,距肿物边缘大于2 cm的正常口腔黏膜组织作为对照组。应用实时荧光定量PCR法检测两组中miR-762的表达,并采用免疫组化法和Western blotting法检测观察组中Ki67、MMP-2和MMP-9的表达。结果:观察组miR-762的表达水平明显高于对照组。观察组miR-762的表达在有无脉管累犯、有无淋巴结转移和不同TNM分期间差异均有统计学意义,而在不同性别、年龄和分化程度间差异均无统计学意义。miR-762的表达与生存时间密切相关。相关分析显示,miR-762的表达与肿瘤的最大径、Ki67增殖指数、MMP-2和MMP-9的表达均呈正相关。结论: 口腔鳞癌miR-762的表达升高,与病变的形成和进展有关。miR-762对癌细胞增殖有明显的促进作用,对癌细胞转移的调节可能是通过介导MMP-2和MM-9实现的。检测miR-762的表达可预测口腔鳞癌的预后。

Objective: To detect the expression of microRNA-762(miR-762) in oral squamous cell carcinoma, and to explore its relationship with cell proliferation and metastasis. Methods: 62 patients with oral squamous cell carcinoma were collected. Tumor tissues were selected as observation group, normal oral mucosa tissues(>2 cm from the edge of the tumors) were selected as control group. miR-762 was detected by real-time fluorescence quantitative PCR in the two groups. Ki67, MMP-2 and MMP-9 were detected by immunohistochemical method in observation group. Ki67, MMP-2 and MMP-9 were detected by Western blotting method in observation group. Results: The expression of miR-762 was significantly higher in observation group than that in control group. The expression of miR-762 was statistically significant in different vascular recidivism, lymph node metastasis and different TNM stages, but was not statistically significant in gender, age and differentiation in the observation group. The expression of miR-762 was closely related to survival time. Positive correlation was found between miR-762 and maximum diameter, miR-762 and Ki67 proliferation index, miR-762 and MMP-2 expression, miR-762 and MMP-9 expression. Conclusion: The increased expression of miR-762 is related to the formation and progression in oral squamous cell carcinoma. Expression of miR-762 can promote cell proliferation. The metastasis regulation of miR-762 may be mediated by MMP-2 and MM-9. Expression of miR-762 may be related to prognosis.

参考文献:

[1] LIU C J,LIN S C,YANG C C,et al.Exploiting salivary miR-31 as a clinical biomarker of oral squamous cell carcinoma[J].Head Neck,2012,34(2):219-224.
[2] HARADA K,FERDOUS T,MINAMI H,et al.Prognostic significance of FOXM1 in oral squamous cell carcinoma patients treated by docetaxel-containing regimens[J].Mol Clin Oncol,2019,10(1):29-36.
[3] MO X,LU Y,HAN J.Effects of targeted modulation of miR-762 on expression of the IFITM5 gene in Saos-2 cells[J].Intractable Rare Dis Res,2014,3(1):12-18.
[4] 张申华,戴婷,宋成文.miR-762对卵巢癌细胞增殖的影响[J].华南国防医学杂志,2017,32(9):582-585.
[5] MUN J,TAM C,CHAN G,et al.microRNA-762 is upregulated in human corneal epithelial cells in response to tear fluid and Pseudomonas aeruginosa antigens and negatively regulates the expression of host defense genes encoding RNase7 and ST2[J].PLoS One,2013,8(2):e57850.
[6] LI Y,HUANG R,WANG L,et al.microRNA-762 promotes breast cancer cell proliferation and invasion by targeting IRF7 expression[J].Cell Prolif,2016,48(6):643-649.
[7] HOU R,YANG Z,WANG S,et al.miR-762 can negatively regulate menin in ovarian cancer[J].Onco Targets Ther,2017,12(10):2127-2137.
[8] 刘爱东,马征,熊艳杰,等.胃腺癌中PYGOPUS2、基质金属蛋白酶-2和-14的表达及对预后的判断价值[J].中国老年学杂志,2016,36(12):2955-2957.
[9] 闫永刚,刘爱东,苏经题,等.基质金属蛋白酶-2和CD105在胃癌中的表达及其相关性的研究[J].临床外科杂志,2013,21(5):352-354.
[10] 樊晓静,史志涛,孙昕.胃癌组织microRNA-762表达及其临床意义[J].中华肿瘤防治杂志,2016,23(16):1072-1075.
[11] YU T,WANG X Y,GONG R G,et al.The expression profile of microRNAs in a model of 7,12-dimethyl-benz[a]anthrance-induced oral carcinogenesis in Syrian hamster[J].J Exp Clin Cancer Res,2009,28(13):64-65.
[12] 李强,陈志刚,任婕,等.结直肠癌中微小RNA-762表达的临床意义及作用机制[J].河北医药,2018,40(23):26-30.
[13] ZHUANG C,JIANG W,HUANG D,et al.Serum miR-21,miR-26a and miR-101 as potential biomarkers of hepatocellular carcinoma[J].Clin Res Hepatol Gastroenterol,2016,40(4):386-396.
[14] SAMAAN S,LICHNER Z,DING Q,et al.Kallikreins are involved in an miRNA network that contributes to prostate cancer progression[J].Biol Chem,2014,395(9):991-1001.

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