Objective: To explore the operating room nursing of robot assisted radical prostatectomy with single-hole bladder inflation.Methods: To summarize the cooperation of robot assisted single-hole transvesical radical prostatectomy in a patient with prostate cancer and rectal cancer after radical abdominal wall permanent fistulation. Under direct vision, a single port channel was established and placed into the bladder to fill with carbon dioxide. The bladder neck was incised along the border of prostate. Seminal vesicles and vas deferens were separated and resected along the prostate capsule. The ligaments of prostate were ligated and the dorsal deep vein complex (DVC) was sutured. The prostate was cut off, the urethra was cut off, and the bladder was sutured.Results: The operation was successful, and the patients were safely sent to the ward for follow-up treatment after recovery. The patients were discharged 8 days after operation.Conclusion: Using the concept of accelerated rehabilitation in clinical practice, the use of a single incision to minimize the stress of trauma, such as minimal postoperative trauma, pain, is conducive to the rapid recovery of patients.