Objective: To detect the expression levels of prostate cancer gene expression marker 1 (PCGEM1) and lncRNA plasmacytoma variant translocation 1 (pvt1) in the serum of patients with asthma (BA) and to explore their clinical significance.Methods: From January 2016 to April 2019, 93 patients with BA diagnosed in our hospital were prospectively selected as the observation group and 90 healthy people as the control group; the levels of lncRNA PCGEM1 and lncRNA PVT1 in serum were detected by real-time fluorescence quantitative PCR (qRT-PCR); the levels of interleukin-6 (IL-6), interleukin-17 (IL-17) and tumor necrosis factor-α (TNF-α) in serum were measured by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the correlations between the expressions of lncRNA PCGEM1, lncRNA PVT1, IL-6, IL-17 and TNF-α in serum of BA patients; receiver operator characteristic curves (ROC) was used to predict the diagnostic values of lncRNA PCGEM1 and lncRNA PVT1 expressions for BA.Results: The expression level of lncRNA PCGEM1 in serum of the observation group was significantly lower than that of the control group (P<0.05), and the expression levels of lncRNA PVT1, IL-6, IL-17 and TNF-α were significantly higher than those in the control group (P<0.05). There was a negative correlation between the expression of lncRNA PCGEM1 and IL-6, IL-17, TNF-α (r =-0.551,-0.686,-0.602, P<0.05), lncRNA PVT1 was positively correlated with the expressions of IL-6, IL-17 and TNF-α (r=0.709, 0.694, 0.637, P<0.05); and there was a negative correlation between pcgem1 and pvt1 (r=-0.634, P<0.05). The area under ROC curve of lncRNA PCGEM1 in BA diagnosis was 0.859, the cutoff value was 0.52, the sensitivity was 89.2%, and the specificity was 70.0%; the area under ROC curve of lncRNA PVT1 in BA diagnosis was 0.892, the truncation value was 2.36, the sensitivity was 82.8%, and the specificity was 85.6%; the area under ROC curve of combined diagnosis in BA diagnosis was 0.949, the cutoff value was 0.47, the sensitivity was 90.3%, and the specificity was 88.9%.Conclusion: The serum lncRNA PCGEM1 of BA patients shows low expression and high lncRNA PVT1 expression, which were negatively correlated and had certain diagnostic value for BA, and the diagnostic value of combined detection was higher. |