Objective:To investigate the relationship between intestinal flora disorder and Th17/Treg immune balance and secretion of inflammatory cytokines in patients with ulcerative colitis(UC). Methods: The UC patients admitted to our hospital from March 2016 to June 2018 were enrolled as the study subjects. According to the progression of the disease, they were divided into the UC group in the remission period and the UC group in the active period. The healthy subjects in the same period were used as the control group. Real-time quantitative polymerase chain reaction(qRT-PCR) analysis of the number of Enterobacter, Enterococcus, Bacteroides, Bifidobacteria and Lactobacillus in each group. HE staining, immunohistochemistry was used to observe pathological changes and calculate expression levels of inflammatory factors in lesions. Flow cytometry was used to detect the percentages of plasma Treg and Th17 cells. The correlation analysis method was used to analyze the correlation between intestinal flora and inflammatory factors and Treg and Th17 cells.Results: Compared with the control group, the number of Bacteroides, Bifidobacteria and Lactobacillus in the intestinal UC group and the UC group in the active phase decreased, and the number of Enterobacter and Enterococcus increased. The expression of IL-2, IL-6 and IL-17 in the active stage UC and remission stage UC lesion sites was increased, and the expression of IL-10 wasdecreased. The proportion of Treg cells in plasma was decreased, the ratio of Th17 cells and Th17/Treg ratio wereincreased, and the changes in active UC group were more significant. The differences were statistically significant(P<0.05).Pearson's correlation analysis showed that the expression levels of IL-2, IL-6, IL-7 and Th17 cells werenegatively correlated with Bifidobacterium, Lactobacillus and Bacteroides, and positively correlated with Enterococcus and Enterobacter(P<0.05),IL-10 expression level and Treg cell ratio were positively correlated with Bifidobacterium, Lactobacillus and Bacteroides, and negatively correlated with Enterococcus and Enterobacter (P<0.05).Conclusion:Intestinal flora disorder is closely related to the occurrence of UC and the progression of the disease. Intestinal flora disorder may affect the secretion of UC by regulating the secretion of Th17/Treg immune imbalance.
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