网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
LncRNA-GAS5在食管鳞癌中的表达及其对细胞增殖和凋亡的影响
作者:裴家强1  游波1  游凯1  吴蔚2 
单位:1. 中国人民解放军陆军第958医院 心胸外科, 重庆 400020;
2. 陆军军医大学附属西南医院 胸外科, 重庆 400038
关键词:长链非编码核糖核酸生长组织特异性转录体5 PTEN基因 食管鳞癌 增殖 凋亡 
分类号:R735.1
出版年·卷·期(页码):2020·39·第七期(850-855)
摘要:

目的:探讨长链非编码核糖核酸生长组织特异性转录体5(LncRNA-GAS5)在食管鳞癌组织中的表达及其对食管鳞癌细胞株EC9706细胞增殖、凋亡的影响。方法:选取2016年2月至2019年1月于中国人民解放军陆军第958医院诊治的食管鳞癌患者78 例,比较LncRNA-GAS5在食管鳞癌组织及癌旁组织中的表达差异;过表达质粒(pEGFP-C1-GAS5)转染EC9706后通过CCK-8试剂盒以及平板克隆试验检测LncRNA-GAS5过表达对细胞增殖能力的影响,采用Annexin V/PI双染流式细胞术检测LncRNA-GAS5过表达对细胞凋亡的影响,荧光实时定量聚合酶链反应(qRT-PCR)及蛋白质免疫印迹法检测抑癌基因PTEN表达变化。结果:与癌旁组相比,食管鳞癌组LncRNA-GAS5相对表达量降低(P<0.05);与空白组、阴性对照组相比,pEGFP-C1-GAS5组LncRNA-GAS5相对表达量升高(P<0.05);与空白组、阴性对照组相比,pEGFP-C1-GAS5组转染后48、72、96 h OD值、细胞克隆数降低(P<0.05),pEGFP-C1-GAS5组细胞凋亡率升高(P<0.05),PTEN mRNA、PTEN蛋白表达升高(P<0.05)。结论:食管鳞癌患者癌组织内LncRNA-GAS5低表达,LncRNA-GAS5过表达抑制癌细胞增殖,促进癌细胞凋亡,可能是通过促进抑癌基因PTEN表达实现的。

Objective: To investigate the expression of long non-coding ribonucleic acid growth tissue-specific transcript 5 (LncRNA-GAS5) in esophageal squamous cell carcinoma and its effects on the proliferation and apoptosis of EC9706 cells. Methods: 78 patients with esophageal squamous cell carcinoma were selected from February 2016 to January 2019 in 958 Hospital of the Chinese People's Liberation Army, the expression of LncRNA-GAS5 in esophageal squamous cell carcinoma and adjacent tissues was compared; after transfection of EC9706 with pEGFP-C1-GAS5, the effect of over expression of LncRNA-GAS5 on cell proliferation was detected by CCK-8 kit and plate cloning assay, Annexin V/PI double staining flow cytometry was used to detect the effect of overexpression of LncRNA-GAS5 on cell apoptosis, the expression of PTEN was detected by quantitative real time polymerase chain reaction(qRT-PCR) and western blotting. Results: Compared with the adjacent group, the relative expression of LncRNA-GAS5 in esophageal squamous cell carcinoma group was lower (P<0.05); compared with blank group and negative control group, the relative expression of LncRNA-GAS5 in pEGFP-C1-GAS5 group increased (P<0.05); compared with blank group and negative control group, OD value and cell clone number in pEGFP-C1-GAS5 group decreased at 48, 72, 96 hours after transfection (P<0.05), the apoptotic rate of pEGFP-C1-GAS5 group was increased (P<0.05), the expressions of PTEN mRNA and PTEN protein increased (P<0.05). Conclusion: The expression of LncRNA-GAS5 in esophageal squamous cell carcinoma is low, LncRNA-GAS5 overexpression inhibits the proliferation of cancer cells and promotes the apoptosis of cancer cells, probably by promoting the expression of tumor suppressor gene PTEN.

参考文献:

[1] 蒋平,刘德干,翟晨彤,等.胸段食管鳞癌根治术后复发特征的研究[J].现代医学,2018,46(8):876-880.
[2] 杨艳,韦淑贞,杜云翔,等.p53、Ki-67、PCNA的表达与食管鳞癌放疗近期疗效及预后的关系[J].东南大学学报(医学版),2017,36(2):155-161.
[3] 刘阳,赵妍,袁翎,等.食管鳞癌患者预后因素分析[J].中华肿瘤防治杂志,2016,23(2):91-95.
[4] LIN D C,HAO J J,NAGATA Y,et al.Genomic and molecular characterization of esophageal squamous cell carcinoma[J].Nat Genet,2014,46(5):467-473.
[5] 郑伟,董洁,李少华,等.LncRNA-GAS5抑制miR-21介导的非完全匹配靶mRNA降解[J].生物化学与生物物理进展,2017,44(7):580-590.
[6] 胡运清,何方,李卉.长链非编码RNA GAS5在胃腺癌中的表达及生物学意义研究[J].实用医学杂志,2016,32(24):4005-4008.
[7] 中华医学会消化内镜学分会消化系早癌内镜诊断与治疗协作组,中华医学会消化病学分会消化道肿瘤协作组,中华医学会消化病学分会消化病理学组.中国早期食管鳞状细胞癌及癌前病变筛查与诊治共识(2015年,北京)[J].中华消化内镜杂志,2016,33(1):3-18.
[8] 卫美蓉,闻丽芬,李玙.长链非编码RNA与肿瘤相关性的研究进展[J].肿瘤学杂志,2017,23(6):535-540.
[9] LI W,ZHAI L,WANG H,et al.Downregulation of LncRNA GAS5 causes trastuzumab resistance in breast cancer[J].Oncotarget,2016,7(19):27778-27786.
[10] XUE D,ZHOU C,LU H,et al.LncRNA GAS5 inhibits proliferation and progression of prostate cancer by targeting miR-103 through AKT/mTOR signaling pathway[J].Tumor Biol,2016,37(12):16187-16197.
[11] PICKARD M R,WILLIAMS G T.Regulation of apoptosis by long non-coding RNA GAS5 in breast cancer cells:implications for chemotherapy[J].Breast Cancer Res Treat,2014,145(2):359-370.
[12] SUN M,JIN F Y,XIA R,et al.Decreased expression of long noncoding RNA GAS5 indicates a poor prognosis and promotes cell proliferation in gastric cancer[J].BMC Cancer,2014,14:319.
[13] 万金华,陈会平.长链非编码RNA GAS5在卵巢癌中的表达及对肿瘤侵袭的影响[J].实用医学杂志,2016,32(24):4062-4065.
[14] YACQUB-USMAN K,PICKARD M R,WILLIAMS G T.Reciprocal regulation of GAS5 lncRNA levels and mTOR inhibitor action in prostate cancer cells[J].Prostate,2015,75(7):693-705.
[15] CHENG C Y,HU C C,YANG H J,et al.Inhibitory effects of scutellarein on proliferation of human lung cancer A549 cells through ERK and NFκB mediated by the EGFR pathway[J].Chin J Physiol,2014,57(4):182-187.
[16] LIU J J,LIN M,YU J Y,et al.Targeting apoptotic and autophagic pathways for cancer therapeutics[J].Cancer Lett,2011,300(2):105-114.
[17] GUO C,SONG W Q,SUN P,et al.LncRNA-GAS5 induces PTEN expression through inhibiting miR-103 in endometrial cancer cells[J].J Biomed Sci,2015,22:100.
[18] CHIA Y C,CATIMEL B,LIO D S,et al.The C-terminal tail inhibitory phosphorylation sites of PTEN regulate its intrinsic catalytic activity and the kinetics of its binding to phosphatidylinositol-4,5-bisphosphate[J].Arch Biochem Biophys,2015,587(1):48-60.
[19] WANG L,CHO Y L,TANG Y,et al.PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy[J].Cell Res,2018,28(8):787-802.
[20] LI P,MAO W M,ZHENG Z G,et al.Down-regulation of PTEN expression modulated by dysregulated miR-21 contributes to the progression of esophageal cancer[J].Dig Dis Sci,2013,58(12):3483-3493.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 382114 位访问者


 ©《现代医学》编辑部
联系电话:025-83272481;83272479
电子邮件: xdyx@pub.seu.edu.cn

苏ICP备09058541