Objective:To observe the relationship between microRNA-30c(miR-30c) and renal interstitial fibrosis in patients with diabetic nephropathy(DN). Methods:60 patients with Type 2 diabetes(T2DM)and 60 patients with DN were selected in this study, Another 40 healthy people were used as control. The expression of miR-30c was determined by fluorescent quantitative polymerase chain reaction analysis. The TGF-β expression was detected by ELISA analysis. The HA, PC Ⅲ, and Ⅳ-C expression was detected by chemiluminescence analysis.Results: The 2-ΔΔCt values of miR-30c in control group, T2DM group, mild DN group and moderate to severe DN group was 0.62±0.11, 0.47±0.08, 0.32±0.06, and 0.25±0.04. The 2-ΔΔCt values in DN group was lower than that in control and T2DM group(P<0.01), and in moderate to severe DN group was lower than that in mild DN group(P<0.05). The expression of TGF-β, HA, PC Ⅲ, and Ⅳ-C in DN group was higher than that in control and T2DM group(P<0.01), and in moderate to severe DN group was higher than that in mild DN group(P<0.05). The 2-ΔΔCt values of miR-30c was negatively related with TGF-β, HA, PC Ⅲ, and Ⅳ-C expression(P<0.05). ROCanalysis showed that miR-30c expression had predictive value for DN.Conclusion: The low expression of miR-30c is involved in the process of renal interstitial fibrosis in DN. It may be a molecular target for DN diagnosis, disease assessment and clinical treatment.
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