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糖尿病肾病患者外周血微小RNA-30c表达与肾间质纤维化的关系
作者:陈娟  唐文庄  张瑞城  孙嵘嵘 
单位:海南医学院第一附属医院 血液净化科, 海南 海口 570206
关键词:糖尿病肾病 微小RNA-30c 肾间质纤维化 
分类号:R587.2
出版年·卷·期(页码):2020·39·第七期(813-816)
摘要:

目的:观察糖尿病肾病(DN)患者外周血微小RNA-30c(miR-30c)表达变化,探讨其与肾间质纤维化的关系。方法:选取2018年2月至2019年4月我院诊疗的单纯2型糖尿病(T2DM)患者60例和DN患者60例作为研究对象,另取我院健康体检者40例作为对照。荧光定量聚合酶链反应检测miR-30c表达,酶联免疫吸附法(ELISA)测定转化生长因子-β(TGF-β),化学发光法测定透明质酸酶(HA)、Ⅲ型前胶原(PC Ⅲ)、Ⅳ型胶原(Ⅳ-C)。结果:对照组、T2DM组、轻度DN组和中重度DN组miR-30c表达的2-ΔΔCt值分别为0.62±0.11、0.47±0.08、0.32±0.06和0.25±0.04,各组间差异具有统计学意义(F=5.42,P=0.03)。其中DN组(含轻度DN组和中重度DN组)低于对照组和T2DM组(t=6.14,P<0.001;t=6.55,P<0.001),中重度DN组低于轻度DN组(t=3.16,P=0.04)。轻度和中重度DN组TGF-β、HA、PC Ⅲ和Ⅳ-C纤维化指标高于对照组和T2DM组,中重度DN组TGF-β、HA、PC Ⅲ和Ⅳ-C高于轻度DN组,差异均有统计学意义(P<0.05)。DN患者miR-30c表达与TGF-β(r=-0.62,P<0.05)、HA(r=-0.53,P<0.05)、PC Ⅲ(r=-0.60,P<0.05)及Ⅳ-C(r=-0.58,P<0.05)表达水平呈负相关。受试者工作特征曲线(ROC曲线)分析显示 miR-30c表达水平对 DN有预测价值。结论:miR-30c低表达参与了DN肾间质纤维化进程,其有可能成为DN诊断、病情评估的分子标志物以及临床治疗的分子靶点。

Objective:To observe the relationship between microRNA-30c(miR-30c) and renal interstitial fibrosis in patients with diabetic nephropathy(DN). Methods:60 patients with Type 2 diabetes(T2DM)and 60 patients with DN were selected in this study, Another 40 healthy people were used as control. The expression of miR-30c was determined by fluorescent quantitative polymerase chain reaction analysis. The TGF-β expression was detected by ELISA analysis. The HA, PC Ⅲ, and Ⅳ-C expression was detected by chemiluminescence analysis.Results: The 2-ΔΔCt values of miR-30c in control group, T2DM group, mild DN group and moderate to severe DN group was 0.62±0.11, 0.47±0.08, 0.32±0.06, and 0.25±0.04. The 2-ΔΔCt values in DN group was lower than that in control and T2DM group(P<0.01), and in moderate to severe DN group was lower than that in mild DN group(P<0.05). The expression of TGF-β, HA, PC Ⅲ, and Ⅳ-C in DN group was higher than that in control and T2DM group(P<0.01), and in moderate to severe DN group was higher than that in mild DN group(P<0.05). The 2-ΔΔCt values of miR-30c was negatively related with TGF-β, HA, PC Ⅲ, and Ⅳ-C expression(P<0.05). ROCanalysis showed that miR-30c expression had predictive value for DN.Conclusion: The low expression of miR-30c is involved in the process of renal interstitial fibrosis in DN. It may be a molecular target for DN diagnosis, disease assessment and clinical treatment.

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