miR-145通过调节细胞周期相关蛋白表达水平抑制乳腺癌MCF-7细胞增殖的机制研究-现代医学

miR-145通过调节细胞周期相关蛋白表达水平抑制乳腺癌MCF-7细胞增殖的机制研究

单位：1. 邯郸市第二医院肿瘤内科, 河北邯郸 056001;
2. 邯郸市第二医院胸外科, 河北邯郸 056001;
3. 磁县肿瘤医院胸外科, 河北磁县 056500

分类号：R737.9

出版年·卷·期（页码）：2020·39·第六期（719-723）

摘要：

目的： 探索微小RNA（miR-145）对乳腺癌人巨噬细胞趋化因子（MCF-7）细胞增殖的抑制作用及miR-145抑制乳腺癌细胞增殖的具体分子机制。方法： 定量聚合酶链反应实验检测miR-145在转染试剂RNAiMAX中的转染效率。细胞计数试剂盒（CCK）-8实验检测miR-145过表达后，乳腺癌人巨噬细胞趋化因子（MCF）-7细胞的增殖曲线。流式细胞术检测过表达miR-145对乳腺癌MCF-7细胞周期分布的影响。蛋白质印记法检测过表达miR-145对乳腺癌MCF-7细胞周期相关蛋白P21、细胞周期蛋白cyclin D1表达的调控作用。结果： RNAiMAX能够有效转染miR-145进入乳腺癌MCF-7细胞内，且转染组miR-145表达量显著高于对照组细胞（P<0.05）。CCK-8实验结果表明过表达miR-145能够显著抑制乳腺癌MCF-7细胞的增殖（P<0.05）。流式细胞实验结果表明过表达miR-145能够显著提高乳腺癌MCF-7细胞在G0/G1期的比例。蛋白质印记法检测结果显示过表达miR-145能够降低周期相关蛋白P21、cyclin D1的表达水平。结论： 研究结果表明miR-145能够抑制乳腺癌MCF-7细胞的增殖，这种抑制与细胞周期相关蛋白P21、cyclin D1的表达水平降低相关，该结果使细胞周期停滞于G0/G1期，进而抑制肿瘤发展。

Objective: To investigate the inhibiting effect of microRNA(miR)-145 on the proliferation of macrophage chemotactic factor 7(MCF-7) cells in breast cancer, and to test the molecular mechanism of miR-145 inhibiting the proliferation of cancer cells.Methods: The transfection of miR-145 by RNAiMAX was detected by quantitative polymerase chain reaction. The proliferation result of breast cancer MCF-7 cells was detected by cell countingkit-8 (CCK-8) assay after overexpression of miR-145. The effect of overexpression of miR-145 on the cell cycle distribution of MCF-7 cells was examined by flow cytometry. The regulation of overexpression of miR-145 on the expression of cell cycle-associated proteins P21 and Cyclin D1 in breast cancer MCF-7 cells were detected by western blot.Results: RNAiMAX was able to efficiently transfect miR-145 into MCF-7 cancer cells. The expression of miR-145 in the transfected group was significantly higher than control group (P<0.05). CCK-8 results indicated that overexpression of miR-145 significantly inhibited the proliferation of MCF-7 cells (P<0.05). Flow cytometry results showed that overexpression of miR-145 significantly increased the proportion of G0/G1 phase in MCF-7 cells. Western blot results indicated that overexpression of miR-145 reduced the expression levels of the cycle-associated proteins P21 and Cyclin D1.Conclusion: The results indicate that miR-145 inhibits the proliferation of MCF-7 cells, and miR-145 arrest the cancer cells at G0/G1 stage in cell cycle by attenuating the expression levels of the cycle-associated proteins P21 and cyclin D1.

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