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HSP70、P53及β-Catenin在肠道息肉恶变中的表达及相关性
作者:沈晓东1  宋文2  韩晓东3 
单位:1. 沈阳二四二医院 急诊科, 辽宁 沈阳 110034;
2. 沈阳二四二医院 病理科, 辽宁 沈阳 110034;
3. 沈阳二四二医院 普通外科, 辽宁 沈阳 110034
关键词:家族性腺瘤性息肉病 热休克蛋白70 P53 β-Catenin 
分类号:R735.3
出版年·卷·期(页码):2020·39·第一期(39-44)
摘要:

目的: 研究热休克蛋白70(Heat shock protein 70,HSP70)、P53及β-Catenin在家族性腺瘤性息肉病(familial adenomatous polyposis,FAP)肠道息肉恶变中的表达及相关性。方法: 采集6周龄及9周龄APCΔ716/+小鼠的肠道标本,通过免疫组织化学染色、蛋白质免疫印迹分析及RT-PCR等实验方法明确良性息肉及息肉恶变组织中HSP70、P53及β-Catenin的表达及相关性。方法: 肠道息肉恶变组织中HSP70及P53免疫组织化学染色的平均光密度较6周小鼠良性息肉显著增加(P均 < 0.001),HSP70及P53的蛋白质免疫印迹分析条带较良性息肉明显增加,HSP70及P53的mRNA表达也较良性息肉显著增强(P均 < 0.001);但息肉恶变组织β-Catenin的平均光密度及mRNA与良性息肉比较虽然略有升高,但未见显著差异(P=0.076,P=0.091)。HSP70 和P53 在息肉恶变组织中的表达存在明显的正相关(r=0.731,P=0.007),但HSP70与β-Catenin的表达未见明显相关性。方法: FAP肠道息肉恶变组织中HSP70及P53表达均较良性息肉显著升高,其表达具有呈明显的正相关,提示HSP70可能通过与P53相互作用,促进FAP的肠道息肉恶变。该发现为HSP70应用于抑制FAP肠道息肉恶变提供重要的理论依据。

Objective: To investigate the expression and correlation of Heat shock protein 70(HSP70), P53 and β-Catenin in malignant polyps in familial adenomatous polyposis (FAP). Methods: The intestinal specimens of APCΔ716/+mice were collected at 6 weeks and 9 weeks. Immunohistochemical staining, western blot and RT-PCR were performed to determine the expression and correlation of HSP70, P53 and β-Catenin in malignant polyps. Results: The average light densities of HSP70 and P53 in malignant polyps were significantly higher than those of benign polyps (P all<0.001) in immunohistochemical staining, and the strips of HSP70 and P53 in malignant polyps were significantly stronger than those in benign polyps in western blot analysis. The mRNA expressions of HSP70 and P53 in malignant polyps were significantly higher than those of benign polyps (P all<0.001). However, although the average light density and mRNA of β-Catenin in malignant polyps increased slightly, there were no significant difference compared with benign polyps (P=0.076, P=0.091). The expressions of HSP70 and P53 protein showed a positive correlation (r=0.731,P=0.007) in malignant polyps, while there was no obvious correlation between the expressions of HSP70 and β-Catenin. Conclusion: The expressions of HSP70 and P53 in malignant polyps of FAP are significantly higher than those in benign polyps, and there is a positive correlation between the expressions of HSP70 and P53 proteins in malignant polyps. HSP70 may promote the carcinogenesis of benign polyps in FAP through the interaction with P53. These findings provide an important theoretical basis for the application of HSP70 in FAP preventing intestinal polyps from occurring

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