Objective: To explore and analyze the correlation between the expression level of aromatic hydrocarbon receptor (AhR) protein and embryonic diapause. Methods: From June 2016 to June 2017, 50 pregnant women with embryonic diapause were selected as observation group, at the same time, 50 pregnant women who did not have embryonic diapause were selected into control group. The expression of AhR in peripheral blood and chorionic villi of the two groups was observed and recorded, and the related factors affecting embryo sterilization were analyzed by univariate variance analysis and Logistic regression analysis, and the correlation between each factor and embryo diapauce was analyzed. Results: Compared with the control group, the expression of AhR in the peripheral blood and villus tissues of the observation group was significantly higher than that of the control group (P<0.05). The results of univariate variance analysis showed that the in vitro fertilization-embryo transfer history, the history of pelvic inflammatory disease, and the history of abortion were significantly different for the incidence of embryonic diapause(P<0.05). At the same time, the incidence of embryonic diapause of different concentrations of peripheral blood AhR protein and different expression of villous tissue AhR mRNA and protein was significantly different (P<0.05); Logistic regression analysis showed that there was a correlation between the history of pelvic inflammation, induced abortion, AhR protein in peripheral blood and AhR mRNA, chorionic villi AhR protein in chorionic villi (P<0.05). Conclusion: The history of pelvic inflammatory disease, the history of abortion, the AhR protein of peripheral blood, the AhR mRNA of villus tissue, and the AhR protein of villous tissue are significantly correlated with the embryonic diapause, which can provide reference value for clinical diagnosis of embryonic diapause. |
[1] 王云凤,连立芬,侯海燕,等.不明原因胚胎停育绒毛和蜕膜组织芳香烃受体和细胞色素P4501A1蛋白的表达及意义[J].国际生殖健康/计划生育杂志,2017,36(6):451-453,473.
[2] YANG L,ZHANG B,YUAN Y,et al.Oxidative stress and DNA damage in utero and embryo implantation of mice exposed to carbon disulfide at peri-implantation.[J].Human and Experimental Toxicology,2014,33(4):424-434.
[3] 郝克红.芳香烃受体在胎盘组织的表达及其对胎盘滋养层细胞增殖的影响[D].上海:复旦大学,2012.
[4] SONG S H,LIM J J,BANG J K,et al.Evaluation of sperm deoxyribonucleic acid (DNA) damage and effects on embryo development using a mouse cryptorchidism model[J].Urology,2013,82(3):743-743.
[5] ZHANG B,SHEN C,YANG L,et al.DNA damage and apoptosis of endometrial cells cause loss of the early embryo in mice exposed to carbon disulfide[J].Toxicology and Applied Pharmacology,2013,273(2):381-389.
[6] 楼姣英,余碧波,彭朋,等.胚胎停育危险因素及中医证候、体质类型的初探[J].中日友好医院学报,2017,31(5):297-298,303.
[7] 谢幸,苟文丽.妇产科学[M].8版.北京:人民卫生出版社,2013:124-125.
[8] 侯雪凤,张晶,姚海凤,等.寿光地区胚胎停育的环境影响因素分析[J].临床医学,2015,13(4):42-43,44.
[9] 郭立燕,翟敏,王莎莎,等.胚胎停育影响因素的Meta分析[J].中国儿童保健杂志,2016,24(2):166-169.
[10] 郭立燕,翟敏,毛书奇,等.胚胎停育环境行为危险因素的病例对照研究[J].环境与健康杂志,2017,34(4):341-344.
[11] ELERSEK T, PLAZAR J,FILIPIC M.A method for the assessment of DNA damage in individual,one day old,zebrafish embryo (Danio rerio),without prior cell isolation[J].Toxicology in vitro:an international journal published in association with BIBRA,2013,27(8):2156-2159.
[12] ANCHORDOQUY J P,ANCHORDOQUY J M,SIRINI M A,et al.Effect of different manganese concentrations during in vitro maturation of bovine oocytes on DNA integrity of cumulus cells and subsequent embryo development.[J].Reproduction in Domestic Animals,2013,48(6):905-911.
[13] 赖智双,陈银娟.孕妇胚胎停育危险因素的病例对照研究[J].中国保健营养(下旬刊),2013,23(11):6361-6362.
[14] 张晶,侯雪凤,姚海凤,等.环境因素对胚胎停育的影响因素分析[J].首都食品与医药,2015,21(18):16-17.
[15] 丁瑛,吴维光.不明原因胚胎停育患者外周血及绒毛组织中芳香烃受体的表达及意义[J].国际妇产科学杂志,2017,44(6):626-628. |