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实时荧光定量PCR检测结肠癌中HER2和TOPOⅡα mRNA的表达
作者:蒋林哲1  王月华2  王立波3  鞠晓红2  钟秀宏4 
单位:1. 吉林省吉林市人民医院 普通外科治疗中心, 吉林 吉林 132001;
2. 吉林省吉林医药学院 病原技术教研室, 吉林 吉林 132013;
3. 吉林省吉林市人民医院 病理科, 吉林 吉林 132001;
4. 吉林省吉林医药学院 病理教研室, 吉林 吉林 132013
关键词:HER2 TOPOⅡα 结肠癌 实时荧光定量PCR 
分类号:R735.3+5
出版年·卷·期(页码):2020·39·第一期(26-30)
摘要:

目的: 探讨HER2和TOPOⅡα mRNA在结肠癌组织中的基因表达水平。方法: 应用实时荧光相对定量PCR的方法检测30例结肠癌组织、癌旁组织和远端正常结肠组织中HER2和TOPOⅡα mRNA 的表达,用2-ΔΔCT的方法计算相对于远端正常组织结肠癌组织中HER2和TOPOⅡα mRNA的表达倍数。方法: 经实时荧光定量PCR法检测,结肠癌组织中HER2和TOPOⅡα的表达与癌旁组织和远端正常结肠组织相比均有显著性差异(P<0.01),HER2和TOPOⅡα相对表达量分别是远端正常结肠组织的8.00倍和 2.00倍(2.95~10.41,0.98~3.14)。HER2和TOPOⅡα mRNA在结肠癌组织中的表达呈正相关,而在癌旁组织和远端正常结肠组织HER2和TOPOⅡα mRNA表达未见相关性。方法: HER2和TOPOⅡα mRNA在结肠癌组织中的高表达提示HER2和TOPOⅡα可能在结肠癌的发生发展中起着一定的作用,两者表达相关性提示联合两者检测对结肠癌患者的诊断、靶向治疗和预后评估具有临床指导意义。

Objective: To investigate the gene expression level of HER2 and TOPO Ⅱ α mRNA in colon cancer. Methods: The expression of HER2 and TOPO Ⅱ α mRNA in 30 cases of colon cancer, adjacent tissues and distal normal tissues were detected by real-time fluorescence relative quantitative PCR. The expression of HER2 and TOPO Ⅱ α mRNA was calculated by 2-ΔΔCT. Results: The expression of HER2 and TOPO Ⅱ α in colon cancer tissues was significantly different from that in adjacent tissues and distal normal colon tissues (P<0.01). The relative expression of HER2 and TOPO Ⅱ α was 8.00 times and 2.00 times (2.95~10.41, 0.98~3.14) of that in distal normal colon tissues, respectively. There was a positive correlation between HER2 and TOPO Ⅱ α mRNA expression in colon cancer, but no correlation between HER2 and TOPO Ⅱ α mRNA expression in adjacent and distal normal colon tissues. Conclusion: The high expression of HER2 and TOPO Ⅱ α mRNA in colon cancer suggests that HER2 and TOPO Ⅱ α may play a role in the occurrence and development of colon cancer. The correlation between HER2 and TOPO Ⅱ α suggests that the combination of the two detection has clinical significance in the diagnosis, targeted treatment and prognosis evaluation of colon cancer patients.

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