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卵巢上皮性癌组织中的Pax-5和miR-215表达水平及临床意义
作者:马会1  谢双双1  于雪娟1  李芳1  高娟2 
单位:1. 河北北方学院附属第一医院 妇产科, 河北 张家口 075000;
2. 邯郸市妇幼保健院 妇产科, 河北 邯郸 056002
关键词:卵巢上皮性癌 Pax-5 miR-215 临床病理特征 预后 
分类号:R737.31
出版年·卷·期(页码):2020·39·第一期(15-20)
摘要:

目的: 研究Pax-5和miR-215在卵巢上皮性癌组织中的表达水平,并探讨其与患者预后的关系。方法: 收集行手术切除的卵巢上皮性癌组织和正常卵巢上皮组织各80例,采用实时荧光定量PCR(RT-PCR)法检测组织中Pax-5和miR-215表达水平,采用Pearson相关系数(r)法分析Pax-5和miR-215的相关性,并分析Pax-5、miR-215表达与卵巢上皮性癌临床病理特征的关系;采用Kaplan-Meier(K-M)生存曲线分析Pax-5和miR-215不同表达水平对患者无瘤生存率的影响,COX回归模型用于分析影响卵巢上皮性癌无瘤生存期的危险因素。方法: 卵巢上皮性癌组织中Pax-5和miR-215表达水平均低于正常卵巢上皮组织(1.39±0.36)vs(2.03±0.54)、(0.92±0.27)vs(1.36±0.41),差异有统计学意义(P < 0.05);卵巢上皮性癌组织中Pax-5和miR-215呈正相关(r=0.677,P=0.010);Pax-5表达水平与卵巢上皮性癌淋巴结转移(1.30±0.29) vs (1.53±0.33)、肿瘤大小(1.32±0.25) vs (1.46±0.31)和术后复发情况(1.29±0.18) vs (1.44±0.32)有关(P < 0.05),miR-215与卵巢上皮性癌FIGO分期(0.97±0.20) vs (0.85±0.23)、淋巴结转移(0.83±0.19) vs (1.06±0.24)、肿瘤大小(0.86±0.20) vs (0.97±0.21)和术后复发情况(0.79±0.18) vs (0.98±0.23)有关(P < 0.05);Pax-5高表达组患者无瘤生存率高于Pax-5低表达组(P < 0.05),miR-215高表达组患者无瘤生存率高于miR-215低表达组(P < 0.05);淋巴结转移、肿瘤大小、术后复发情况和肿瘤组织中Pax-5、miR-215表达水平是影响卵巢上皮性癌术后无瘤生存期的独立危险因素(OR=3.792,3.309,4.276,2.692,2.090,P均 < 0.05)。方法: Pax-5和miR-215在卵巢上皮性癌组织中表达下调,与卵巢上皮性癌增殖、转移等恶性行为及不良预后有关。

Objective: To investigate the expressions of Pax-5 and miR-215 in epithelial ovarian cancer tissues and their relationships with prognosis. Methods: 80 cases of epithelial ovarian cancer tissues and normal epithelial tissues were collected, the expression levels of Pax-5 and miR-215 in tissues were detected by real-time fluorescence quantitative PCR (RT-PCR). Pearson correlation coefficient (r) method was used to analyze the correlation between Pax-5 and microRNA-215 in epithelial ovarian cancer and their relationships with clinicopathological characteristics. Kaplan-Meier (K-M) survival curve was used to analyze the effect of Pax-5 and miR-215 expression levels on the tumor-free survival rate of patients. COX regression model was used to analyze the risk factors affecting the tumor-free survival of epithelial ovarian cancer. Results: The expression levels of Pax-5 and miR-215 in epithelial ovarian cancer tissues were lower than those in normal ovarian epithelial tissues (1.39±0.36) vs(2.03±0.54),(0.92±0.27) vs (1.36±0.41), the difference was significant (P<0.05); Pax-5 and miR-215 were positively correlated in epithelial ovarian cancer(r=0.677, P=0.010); the expression of Pax-5 was correlated with lymph node metastasis(1.30±0.29) vs (1.53±0.33), tumor size (1.32±0.25) vs (1.46±0.31) and postoperative recurrence (1.29±0.18) vs (1.44±0.32) in epithelial ovarian cancer (P<0.05), the expressions of miR-215 was correlated with FIGO stage (0.97±0.20) vs (0.85±0.23), lymph node metastasis (0.83±0.19) vs (1.06±0.24), tumor size (0.86±0.20) vs (0.97±0.21) and postoperative recurrence(0.79±0.18) vs (0.98±0.23) (P<0.05); the tumor-free survival rate of patients with higher expression of Pax-5 was higher than those with lower expression of Pax-5 (P<0.05), the tumor-free survival rate of patients with higher expression of miR-215 was higher than those of lower expression of miR-215 (P<0.05); lymph node metastasis, tumor size, postoperative recurrence and the expressions of Pax-5 and miR-215 were independent risk factors for postoperative tumor-free survival after epithelial ovarian cancer surgery (OR=3.792, 3.309, 4.276, 2.692, 2.090, P<0.05). Conclusion: The expressions of Pax-5 and miR-215 are down-regulated in epithelial ovarian cancer tissue, which is related to malignant behaviors such as proliferation, metastasis and poor prognosis of epithelial ovarian cancer.

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