Objective: To studythe effect of different doses of pomegranate seed oil(PSO) on chondral and subchondral bone elements calcium(Ca),phosphorus(P) content in the condyle cartilage layer of femur and serum alkaline phosphatase(ALP),peroxidase dismutase(SOD) activity and malondialdehyde(MDA) content in osteoarthritis(OA)rats, and reveal the experimental basis for promoting cartilage repairation and inhibiting joint degeneration. Methods: Experimental animals were divided into 5 groups:blank group, model control group, low dose group(experimental group 1), middle dose group(experimental group 2) and high dose group(experimental group 3). Rats in the blank group were performed sham operations, rats in the other four groups wereestablished OA models by the classic Hulth method.After driven 4 weeks,two rats were randomly selected from each group (except the blank group) and killed with broken necks, and MicroCT parameter analysis were used to confirm the models were successful. Rats in 5 groups were fed withnormal diet, besides,rats in the model control group were fed with 1.59 g·kg-1 pure corn oil by gavage every day,rats in the experimental group 1,2,3 were fed with 0.95, 1.27 and 1.59 g·kg-1 pure PSO by gavage. Ratsin each group were fed under the same conditions, and were allowed to drink and eat freely. All the rats were weighed every 7 days to adjust the dose of PSO. Continue feeding for 12 weeks, the rats were injected intraperitoneally with 1% pentobarbital sodium at 90 mg·kg-1 to anesthetize them to death, and 6 ml abdominal venous blood was taken and centrifugated with 3 000 r·min-1 for 10 min. The levels of serum MDA and the activity of ALP, SOD were measured with an atomic absorption spectrophotometer. The femoral condyle specimens were cut longitudinally, and after drying, the sections were observed with EDX 4500H energy dispersive X-ray fluorescence spectrometer and JEM-400 0EX energy selective electron microscope. Ca and P contents were calculated according to the absorption spectra of the elements on the electron beam.Results: There was no significant difference in the content of Ca and P in cartilage and subchondral bone and the activity of MDA, ALP and SOD in serum between the model control group and the blank group after corn oil intervention (P>0.05).After PSO intervention, the other indexes of the three experimental groups were lower than those of the model control group except for the increase of SOD activity,the difference was statistically significant(P<0.05). Compared with experimental group1, the content of Ca and the activity of MDA in serum decreased while the activity of SOD increased in experimental group 2,3,the difference was statistically significant(P<0.05). Conlusion: Corn oil has no inhibitory effect on OA cartilage degeneration and subchondral osteosclerosis. PSO can effectively reduce the content of Ca and P in cartilage and subchondral bone of OA rats, weaken the activity of cartilage transformation, inhibit the cartilage degeneration, subchondral bone sclerosis and proliferation. At the same time, PSO can reduce the activity of ALP, suggesting that it has estrogen activity, can block the increase of ALP activity and improve the high transformation state of cartilage. In addition, PSO can effectively reduce the activity of MDA,has quenching effect onoxygen free radicals.
 HUANG Z,GUO W B,WANG X H,et al.Advances in the pathogenesis of chronic synovitis in early osteoarthritis[J].Pra dics,2016,22(5):427-428.
 LIU Y,LIU H,LI M,et al.Association of farnesyl diphosphate synthase polymorphisms and response to alendronate treatment in Chinese postmenopausal women with osteoporosis[J].Chin Med J(Engl),2014,127(4):662-801.
 KAUFMAN M,KOHNO,MURASE J,et al.Delta12-oleate desaturase-related enzymes associated with formation of conjugated trans-delta11,cisdelta13 double bonds[J].J Biol Chem,2003,278:4603-4610.
 SUANTAWEE T,TANTAVISUT S,ADISAKWATTANA S,et al.Oxidative stress,vitamine,and antioxidant capacity in knee osteoarthritis[J].J Clin Diagn Res,2013,7:1857-1858.
 REGAN E A,BOWLER R P,CRAPO J D,et al.Joint fluid antioxidants are decreased in osteoarthritic joints compared to joints with macroscopically intact cartilage and subacute injury[J].Osteoarthritis Cartilage,2008,16:515-521.
 SEO S K,YANG H I,LIM K J,et al.Changes in serum levels of cartilage oligomeric matrix protein after estrogen and alendronate therapy in postmenopausal women[J].Gyn Obste Inves,2012,47(31):352-353.
 WU X Z,GAO X H,YANG F H.Effects of dietary copper on organ indexes,tissular Cu,Zn and Fe deposition and fur quality of growing-furring male mink[J].Jou Ani S Techn,2015,57:6.
 GOL W J.Scanning electron microscopy technique X-ray microanalysis[J].Bone Leng Ske,2008,25(6):321-324.