目的：探索转化生长因子-β（transforming growth factor-β，TGF-β）是否能够通过上调细胞趋化因子受体4（CXCR4）的表达从而促进肝癌细胞增殖的作用机制，并证实该效应是否与其激活smad通路相关。方法：采用蛋白免疫印迹试验检测20 ng·ml^-1的TGF-β作用于肝细胞癌Huh7细胞后TGF-β、CXCR4、CyclinD1、CDK4和smad通路的蛋白表达水平。使用smad阻断剂LY2109761和TGF-β分别作用于肝癌细胞后，采用蛋白免疫印迹试验检测p-smad1、p-smad2、smad、CXCR4、CyclinD1和CDK4蛋白的表达水平。结果：20 ng·ml^-1的TGF-β显著上调了TGF-β、CylinD1和CDK4的表达水平（P<0.05），同时也显著上调CXCR4、p-smad1和p-smad2的蛋白表达（P<0.05）。smad阻断剂LY2109761显著抑制了TGF-β诱导的smad1和smad2的活化（P<0.05），同时也抑制了CXCR4、CyclinD1和CDK4的表达水平（P<0.05）。结论： TGF-β可能通过激活smad信号通路而上调CXCR4的表达水平，并且上调了细胞周期蛋白CylinD1和CDK4的表达，最终促进了肝癌细胞的增殖反应。

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