不育男性白细胞精液症与精浆IL-6及TNF-α表达水平的相关性分析-现代医学

不育男性白细胞精液症与精浆IL-6及TNF-α表达水平的相关性分析

单位：宝鸡市人民医院泌尿外科, 陕西宝鸡 721000

分类号：R698.2

出版年·卷·期（页码）：2019·38·第十期（1202-1206）

摘要：

目的：探究白细胞精液症不育患者精浆白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）的表达水平与精液白细胞数量之间的相关性。方法：选择2016年3月至2017年10月期间来我院就诊的85例男性不育患者，分为3个观察组：白细胞精液症精液参数正常者为观察1组（27例）、白细胞精液症精液参数异常者为观察2组（30例）、少弱畸精子症不育患者为观察3组（28例）；另选取可育男性20例为对照组。检测各组研究对象性激素水平、精液量、精子密度、精子活动率、精子畸形率、精浆白细胞数量、精浆IL-6和TNF-α的表达、精浆活性氧（ROS）的表达。结果：3个观察组患者的精子总数、精子浓度、精子前向运动率均显著低于对照组（P<0.001），精浆IL-6和TNF-α的表达水平升高（P<0.001）。而白细胞精液症组患者的精浆IL-6和TNF-α的表达水平显著于非白细胞精液症组（P<0.001），精浆ROS水平增高（P<0.05）。精浆IL-6和TNF-α的表达水平与精液白细胞数量呈正相关（r=0.631，P<0.001和r=0.762，P<0.001）。结论：精浆IL-6和TNF-α表达水平的增高与男性不育症之间存在密切关系，精浆IL-6和TNF-α的表达水平与精液白细胞数量的相关性，可作为白细胞精液症患者不育临床诊断和治疗的依据。

Objective:To investigate the relationship between the expression levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in seminal plasma of infertile men with leukocytospermia. Methods:A total of 85 cases of infertile men treated in hospital from March 2016 to October 2017 were divided into three observation groups, i.e., leukocytospermia patients with normal semen parameters(observation group 1,27 cases), leukocytospermia patients with abnormal semen parameters(observation group 2,30 cases) and oligospermia patients(observation group 3,28 cases). Twenty fertile males were selected as control group. The levels of sex hormones, semen volume, sperm density, sperm activity rate, sperm malformation rate, leucocyte numbers in seminal plasma, reactive oxygen species(ROS), IL-6 and TNF-α expression were detected in each group. Results:The total number of sperm, sperm concentration and ratio of sperm forward movement in the three observation groups were significantly lower than those in the control group (P<0.001), and the expression levels of IL-6 and TNF-α in seminal plasma of the observation groups were significantly increased(P<0.05). The expression levels of IL-6 and TNF-α, and the ROS level in seminal plasma of leukocytospermia group were significantly higher than those of non-leukocytospermia group (P<0.05). The expression levels of IL-6 and TNF-α in seminal plasma were positively correlated with the number of leukocytes(r=0.631, P<0.001 and r=0.762, P<0.001).Conclusion:The increased expression levels of IL-6 and TNF-α in seminal plasma are closely associated with male infertility and leucocyte numbers, which can be used as clinical indicators for the diagnosis and treatment of leukocytospermia.

参考文献：

[1] 张剑波,尹国良,徐新蓉,等.不育门诊就诊男性精液质量与影响因素分析[J].生殖医学杂志,2015,24(12):1041-1044.
[2] 张健,许鹏,斯坎达尔,等.显微镜下精索静脉结扎对精子DNA完整性的影响[J].现代泌尿外科杂志,2016,21(4):267-270.
[3] FORD W C.Comments on the release of the 5th edition of the WHO laboratory manual for the examination and processing of human semen[J].Asian J Androl,2010,12(1):59-63.
[4] MICILLO A,VASSALLO M R C,CORDESCHI G,et al.Semen leukocytes and oxidative-dependent DNA damage of spermatozoa in male partners of subfertile couples with no symptoms of genital tract infection[J].Andrology,2016,4(5):808-815.
[5] 张俏忻,肖颖秀,程碧珍.精液白细胞异常增加对精子DNA氧化损伤的影响[J].癌变·畸变·突变,2015,27(5):383-385.
[6] AGHAZARIAN A,STANCIK I,PFLUGER H,et al.Influence of pathogens and moderate leukocytes on seminal interleukin (IL)-6, IL-8,and sperm parameters[J].Int Urol Nephrol,2013,45(2):359-365.
[7] AVENDANO C,FRANCHI A,DURAN H,et al.DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome[J].Fertil Steril,2010,94(2):549-557.
[8] 世界卫生组织.世界卫生组织人类精液检查与处理实验室手册[M].北京:人民卫生出版社,2011:7-9.
[9] 王益鑫,郑菊芬.男性不育研究新进展[J].中华男科学杂志,2006,12(9):771-774.
[10] 莫延利.精液白细胞与男性不育关系研究[D].长春:东北师范大学,2008.
[11] 张俏忻,肖颖秀,程碧珍.白细胞异常增加对精液质量及氧化应激的影响[J].国际检验医学杂志,2016,37(6):726-727.
[12] SIMEONOVA P P,TORIUMI W,KOMMINENI C,et al.Molecular regulation of IL-6 activation by asbestos in lung epithelial cells:role of reactive oxygen species[J].J Immunol,1997,159(8):3921-3928.
[13] LIU J,WANG Q,JI X,et al.Prevalence of Ureaplasma urealyticum,Mycoplasma hominis,Chlamydia trachomatisinfections,and semen quality in infertile and fertile men in China[J].Urology,2014,83(4):795-799.
[14] POLITCH J A,TUCKER L,BOWMAN F P,et al.Concentrations and significance of cytokines and other immunologic factors in semen of healthy fertile men[J].Hum Reprod,2007,22(11):2928-2935.
[15] FRACZEK M,KURPISZ M.Cytokines in the male reproductive tract and their role in infertility disorders[J].J Reprod Immunol,2015,108:98-104.
[16] VILVANATHAN S,KANDASAMY B,JAYACHANDRAN A L,et al.Bacteriospermia and its impact on basic semen parameters among infertile men[J].Interdiscip Perspect Infect Dis,2016,2016:1-6.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录