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不育男性白细胞精液症与精浆IL-6及TNF-α表达水平的相关性分析
作者:刘双宁  罗成  赵波  李颖毅  张辉  毕航  刘玮  康启源 
单位:宝鸡市人民医院 泌尿外科, 陕西 宝鸡 721000
关键词:男性不育 白细胞精液症 白细胞介素-6 肿瘤坏死因子-α 
分类号:R698.2
出版年·卷·期(页码):2019·38·第十期(1202-1206)
摘要:

目的:探究白细胞精液症不育患者精浆白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的表达水平与精液白细胞数量之间的相关性。方法:选择2016年3月至2017年10月期间来我院就诊的85例男性不育患者,分为3个观察组:白细胞精液症精液参数正常者为观察1组(27例)、白细胞精液症精液参数异常者为观察2组(30例)、少弱畸精子症不育患者为观察3组(28例);另选取可育男性20例为对照组。检测各组研究对象性激素水平、精液量、精子密度、精子活动率、精子畸形率、精浆白细胞数量、精浆IL-6和TNF-α的表达、精浆活性氧(ROS)的表达。结果:3个观察组患者的精子总数、精子浓度、精子前向运动率均显著低于对照组(P<0.001),精浆IL-6和TNF-α的表达水平升高(P<0.001)。而白细胞精液症组患者的精浆IL-6和TNF-α的表达水平显著于非白细胞精液症组(P<0.001),精浆ROS水平增高(P<0.05)。精浆IL-6和TNF-α的表达水平与精液白细胞数量呈正相关(r=0.631,P<0.001和r=0.762,P<0.001)。结论:精浆IL-6和TNF-α表达水平的增高与男性不育症之间存在密切关系,精浆IL-6和TNF-α的表达水平与精液白细胞数量的相关性,可作为白细胞精液症患者不育临床诊断和治疗的依据。

Objective:To investigate the relationship between the expression levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in seminal plasma of infertile men with leukocytospermia. Methods:A total of 85 cases of infertile men treated in hospital from March 2016 to October 2017 were divided into three observation groups, i.e., leukocytospermia patients with normal semen parameters(observation group 1,27 cases), leukocytospermia patients with abnormal semen parameters(observation group 2,30 cases) and oligospermia patients(observation group 3,28 cases). Twenty fertile males were selected as control group. The levels of sex hormones, semen volume, sperm density, sperm activity rate, sperm malformation rate, leucocyte numbers in seminal plasma, reactive oxygen species(ROS), IL-6 and TNF-α expression were detected in each group. Results:The total number of sperm, sperm concentration and ratio of sperm forward movement in the three observation groups were significantly lower than those in the control group (P<0.001), and the expression levels of IL-6 and TNF-α in seminal plasma of the observation groups were significantly increased(P<0.05). The expression levels of IL-6 and TNF-α, and the ROS level in seminal plasma of leukocytospermia group were significantly higher than those of non-leukocytospermia group (P<0.05). The expression levels of IL-6 and TNF-α in seminal plasma were positively correlated with the number of leukocytes(r=0.631, P<0.001 and r=0.762, P<0.001).Conclusion:The increased expression levels of IL-6 and TNF-α in seminal plasma are closely associated with male infertility and leucocyte numbers, which can be used as clinical indicators for the diagnosis and treatment of leukocytospermia.

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