网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
IL-8及CXCR1、CXCR2在白内障晶状体上皮细胞中的表达及其意义
作者:程丽娜  宋蔚  闫春妮 
单位:西安市第一医院 眼科, 陕西 西安 710002
关键词:白内障 晶状体上皮细胞 白细胞介素8 人CXC趋化因子受体1 人CXC趋化因子受体2 
分类号:R776.1
出版年·卷·期(页码):2019·38·第十期(1197-1201)
摘要:

目的:研究白细胞介素8(IL-8)及其受体人CXC趋化因子受体(CXCR)1、CXCR2在白内障晶状体上皮细胞中的表达,并探讨其临床意义。方法:白内障晶状体前囊膜组织(病例组)和正常透明晶状体前囊膜组织(正常组)分别来自2016年9月至2018年6月在我院行白内障手术的40例患者和40例角膜移植术供体,采用RT-PCR法检测组织中IL-8、CXCR1、CXCR2 mRNA表达量,免疫组化染色法检测组织中CXCR1、CXCR2阳性表达,分析病例组IL-8与CXCR1、CXCR2的关系及IL-8、CXCR1、CXCR2与组织临床分期的关系。结果:病例组晶状体前囊膜组织中IL-8、CXCR1、CXCR2 mRNA相对表达量均高于正常组(P<0.05);病例组CXCR1、CXCR2阳性表达率分别为62.50%、57.50%,均高于正常组(P<0.05);病例组IL-8与CXCR1、CXCR2均呈显著正相关关系(r=0.806、r=0.750,均P<0.05);病例组不同临床分期组织中IL-8、CXCR1、CXCR2表达水平差异有统计学意义(P<0.05),随晶状体病变程度加重呈显著升高趋势(P<0.05)。结论:IL-8及CXCR1、CXCR2在白内障晶状体上皮细胞中的表达升高,与临床分期密切相关,可能参与白内障晶状体病变过程。

Objective:To study the expressions of interleukin-8(IL-8) and its receptors, human CXC chemokine receptor 1(CXCR1) and human CXC chemokine receptor 2(CXCR2) in cataract lens epithelial cells and to explore their clinical significances.Methods:From September 2016 to June 2018, 40 cases of cataract surgery and 40 cases of corneal transplantation donors were selected. The anterior capsule tissues of cataract(case group) and normal anterior capsule tissue of clear lens(normal group) were obtained from 40 cases of patients undergoing cataract surgery and 40 cases of corneal transplantation donors respectively. The expressions of IL-8, CXCR1 and CXCR2 mRNAs in tissues were detected by RT-PCR, and immunohistochemical staining was used to detect the positive expressions of CXCR1 and CXCR2 in tissues. The relationships between IL-8 and CXCR1, CXCR2, and the relationships between the IL-8, CXCR1 and CXCR2 and the tissue clinical staging were analyzed.Results:The expressions of IL-8, CXCR1 and CXCR2 mRNAs in the anterior capsule tissues of the lens in the case group were higher than those in the normal group(P<0.05). The positive expression rates of CXCR1 and CXCR2 in case group were 62.50% and 57.50%, respectively, which were higher than those in normal group(P<0.05). There were significant positive correlations of IL-8 with CXCR1 and CXCR2 in case group(r=0.806 and r=0.750, all P<0.05). The expression levels of IL-8, CXCR1 and CXCR2 in different clinical stages of the case group were significantly different(P<0.05), showing a significant upward trend with the increase of the severity of lens lesions(P<0.05). Conclusion:The expressions of IL-8, CXCR1 and CXCR2 are increased in cataract lens epithelial cells and closely related to clinical stage,which may be involved in the process of cataract lens lesion.

参考文献:

[1] 刘勇.枣庄市≥ 50岁人群白内障患病率及危险因素分析[J].中华全科医学,2014,12(7):1115-1117.
[2] 张小梅,杨剑锋.长沙市老年性白内障患病率及其影响因素分析[J].湖南师范大学学报(医学版),2015,12(2):40-43.
[3] SHIVAPPA N,HEBERT J R,RASHIDKHANI B,et al.Inflammatory potential of diet is associated with increased odds of cataract in a case-control study from Iran[J].Int J Vitam Nutr Res,2018,87(1-2):1-8.
[4] HA H,DEBNATH B,NEAMATI N.Role of the CXCL8-CXCR1/2 axis in cancer and inflammatory diseases[J].Theranostics,2017,7(6):1543-1588.
[5] 金子夜,王心淼,郜青叶,等.老年性白内障和糖尿病性白内障晶状体上皮细胞的超微结构改变及凋亡相关基因的表达[J].中国临床保健杂志,2015,18(5):483-485.
[6] KILIC F,TREVITHICK J R.Modelling cortical cataractogenesis ⅩⅩⅨ.Calpain proteolysis of lens fodrin in cataract[J].Iubmb Life,2015,45(5):963-978.
[7] 谭其文.老年白内障患者血清及房水抗氧化指标及炎性指标的变化研究[J].海南医学院学报,2013,19(8):1149-1152.
[8] TANG S C,LIAO P Y,HUNG S J,et al.Topical application of glycolic acid suppresses the UVB induced IL-6,IL-8,MCP-1 and COX-2 inflammation by modulating NF-κB signaling pathway in keratinocytes and mice skin[J].J Dermatol Sci,2017,86(3):238-248.
[9] ALHARBI B,HENDRAYANI S F,SILVA G,et al.Let-7b inhibits cancer-promoting effects of breast cancer-associated fibroblasts through IL-8 repression[J].Oncotarget,2018,9(25):17825-17838.
[10] WOLF M,DELGADO M B,JONES S A,et al.Granulocyte chemotactic protein 2 acts via both IL-8 receptors,CXCR1 and CXCR2[J].Eur J Immunol,2015,28(1):164-170.
[11] TANAKA K,YOSHITOMI T,HIRAHARA K.Elucidation of distinct roles of guinea pig CXCR1 and CXCR2 in neutrophil migration toward IL-8 and GROα by specific antibodies[J].Biol Pharm Bull,2017,40(5):729-732.
[12] BERTINI R,BARCELOS L S,BECCAU A R,et al.Receptor binding mode and pharmacological characterization of a potent and selective dual CXCR1/CXCR2 non-competitive allosteric inhibitor[J].Br J Pharmacol,2015,165(2):436-454.
[13] SCHNEBERGER D,GORDON J R,DEVASURE J M,et al.CXCR1/CXCR2 antagonist CXCL8(3-74)K11R/G31P blocks lung inflammation in swine barn dust-instilled mice[J].Pulm Pharmacol Ther,2015,31(5):55-62.
[14] 李瑶,吕德官,陈临溪.IL-8及其受体药物与疾病的研究进展[J].中国药理学通报,2014,30(3):310-314.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 295499 位访问者


 ©《现代医学》编辑部
联系电话:025-83272481;83272479
电子邮件: xdyx@pub.seu.edu.cn

苏ICP备09058541