Objective:To study the expressions of interleukin-8(IL-8) and its receptors, human CXC chemokine receptor 1(CXCR1) and human CXC chemokine receptor 2(CXCR2) in cataract lens epithelial cells and to explore their clinical significances.Methods:From September 2016 to June 2018, 40 cases of cataract surgery and 40 cases of corneal transplantation donors were selected. The anterior capsule tissues of cataract(case group) and normal anterior capsule tissue of clear lens(normal group) were obtained from 40 cases of patients undergoing cataract surgery and 40 cases of corneal transplantation donors respectively. The expressions of IL-8, CXCR1 and CXCR2 mRNAs in tissues were detected by RT-PCR, and immunohistochemical staining was used to detect the positive expressions of CXCR1 and CXCR2 in tissues. The relationships between IL-8 and CXCR1, CXCR2, and the relationships between the IL-8, CXCR1 and CXCR2 and the tissue clinical staging were analyzed.Results:The expressions of IL-8, CXCR1 and CXCR2 mRNAs in the anterior capsule tissues of the lens in the case group were higher than those in the normal group(P<0.05). The positive expression rates of CXCR1 and CXCR2 in case group were 62.50% and 57.50%, respectively, which were higher than those in normal group(P<0.05). There were significant positive correlations of IL-8 with CXCR1 and CXCR2 in case group(r=0.806 and r=0.750, all P<0.05). The expression levels of IL-8, CXCR1 and CXCR2 in different clinical stages of the case group were significantly different(P<0.05), showing a significant upward trend with the increase of the severity of lens lesions(P<0.05). Conclusion:The expressions of IL-8, CXCR1 and CXCR2 are increased in cataract lens epithelial cells and closely related to clinical stage,which may be involved in the process of cataract lens lesion.
 刘勇.枣庄市≥ 50岁人群白内障患病率及危险因素分析[J].中华全科医学,2014,12(7):1115-1117.
 SHIVAPPA N,HEBERT J R,RASHIDKHANI B,et al.Inflammatory potential of diet is associated with increased odds of cataract in a case-control study from Iran[J].Int J Vitam Nutr Res,2018,87(1-2):1-8.
 HA H,DEBNATH B,NEAMATI N.Role of the CXCL8-CXCR1/2 axis in cancer and inflammatory diseases[J].Theranostics,2017,7(6):1543-1588.
 KILIC F,TREVITHICK J R.Modelling cortical cataractogenesis ⅩⅩⅨ.Calpain proteolysis of lens fodrin in cataract[J].Iubmb Life,2015,45(5):963-978.
 TANG S C,LIAO P Y,HUNG S J,et al.Topical application of glycolic acid suppresses the UVB induced IL-6,IL-8,MCP-1 and COX-2 inflammation by modulating NF-κB signaling pathway in keratinocytes and mice skin[J].J Dermatol Sci,2017,86(3):238-248.
 ALHARBI B,HENDRAYANI S F,SILVA G,et al.Let-7b inhibits cancer-promoting effects of breast cancer-associated fibroblasts through IL-8 repression[J].Oncotarget,2018,9(25):17825-17838.
 WOLF M,DELGADO M B,JONES S A,et al.Granulocyte chemotactic protein 2 acts via both IL-8 receptors,CXCR1 and CXCR2[J].Eur J Immunol,2015,28(1):164-170.
 TANAKA K,YOSHITOMI T,HIRAHARA K.Elucidation of distinct roles of guinea pig CXCR1 and CXCR2 in neutrophil migration toward IL-8 and GROα by specific antibodies[J].Biol Pharm Bull,2017,40(5):729-732.
 BERTINI R,BARCELOS L S,BECCAU A R,et al.Receptor binding mode and pharmacological characterization of a potent and selective dual CXCR1/CXCR2 non-competitive allosteric inhibitor[J].Br J Pharmacol,2015,165(2):436-454.
 SCHNEBERGER D,GORDON J R,DEVASURE J M,et al.CXCR1/CXCR2 antagonist CXCL8(3-74)K11R/G31P blocks lung inflammation in swine barn dust-instilled mice[J].Pulm Pharmacol Ther,2015,31(5):55-62.