重度急性胰腺炎患者血清生长素-2和sFlt-1之间的关系及临床意义-现代医学

重度急性胰腺炎患者血清生长素-2和sFlt-1之间的关系及临床意义

单位：1. 常州市第七人民医院检验科, 江苏常州 213000;
2. 常州市第一人民医院检验科, 江苏常州 213000;
3. 上海中医药大学附属市中医医院检验科, 上海 200071

关键词：重症急性胰腺炎血清生长素-2 可溶性fms样酪氨酸激酶-1 临床病理参数诊断

分类号：R576

出版年·卷·期（页码）：2019·47·第八期（1017-1022）

摘要：

目的：检测血清生长素-2（Ang-2）、可溶性fms样酪氨酸激酶-1（sFlt-1）在重度急性胰腺炎（SAP）患者中表达情况并探讨其临床意义。方法：选取本院确诊治疗的109例急性胰腺炎（AP）患者为研究对象，同期健康体检者56例作为对照组。酶联免疫吸附（ELISA）法检测各受试者静脉血血清中Ang-2及sFlt-1蛋白水平，ROC曲线分析二者在SAP诊断中的预测价值。结果：与对照组比较，AP患者血清Ang-2、sFlt-1蛋白水平明显增高（P<0.05）。血清Ang-2、sFlt-1水平诊断AP的ROC曲线下面积（AUC）分别为：0.735、0.681。血清Ang-2水平与AP患者CT分级、APACHEⅡ评分及并发急性肺损伤比例有关（P<0.05）；血清sFlt-1水平与AP患者CT分级、APACHEⅡ评分、胸水积液及并发急性肺损伤比例有关（P<0.05）。与MAP组比较，SAP组胰腺炎患者血清Ang-2、sFlt-1蛋白水平显著增高（P<0.05）。血清Ang-2、sFlt-1对SAP患者诊断的AUC分别为0.783、0.751。SAP患者血清Ang-2与sFlt-1表达呈正相关（r=0.611，P<0.05）。结论：SAP患者血清Ang-2、sFlt-1显著升高，检测AP患者血清Ang-2、sFlt-1水平对SAP疾病诊断有一定应用价值。

Objective:To investigate the relationship between serum angiopoietin-2 (Ang-2) and soluble fms-like tyrosine kinase 1 (sFlt-1) in patients with severe acute pancreatitis and its clinical significance. Methods:A total of 109 patients with acute pancreatitis (AP) diagnosed in our hospital were selected as the research subjects, 56 cases of healthy physical examination as control group at the same time. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of Ang-2 and sFlt-1 in venous blood of subjects. The predictive value of ROC curve analysis in SAP diagnosis was analyzed by ROC curve. Results:Compared with the control group, the serum Ang-2 and sFlt-1 protein levels in AP patients increased significantly (P<0.05). The area under the ROC curve (AUC) of serum Ang-2 and sFlt-1 levels in diagnosing AP was 0.735 and 0.681 respectively. The level of serum Ang-2 was related to the CT classification, APACHE Ⅱ score and the proportion of acute lung injury in AP patients (P<0.05). The level of serum sFlt-1 was related to the CT classification, APACHE Ⅱ score, hydrothorax and the proportion of acute lung injury in patients with AP (P<0.05). Compared with group MAP, serum Ang-2 and sFlt-1 protein levels in group SAP were significantly higher than those in group Ang-2 (P<0.05).The AUC of serum Ang-2 and sFlt-1 for SAP patients was 0.783 and 0.751 respectively. There was a positive correlation between serum Ang-2 and sFlt-1 expression in SAP patients (r=0.611, P<0.05). Conclusion:Serum Ang-2 and sFlt-1 levels in patients with SAP have increased significantly. The detection of serum Ang-2 and sFlt-1 levels in AP patients has certain application value in the diagnosis of SAP disease.

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