Objective:To investigate the effect of TRPA1 silencing on IL-1β-induced chondrocyte inflammatory injury and cartilage matrix degeneration.Methods:Mice chondrocytes ATDC5 were divided into blank control group, IL-1β group, scramble group, siTRPA1 group, IL-1β+scramble group, and IL-1β+siTRPA1 group. Cell viability was evaluated by MTT assay. The mRNA level of TNF-α, IL-6, MMP-13, ADAMTS5, Col Ⅱ and ACAN was detected by qRT-PCR and the protein level of Col Ⅱ, ACAN and p-p38 MAPK was detected by Western blot.Results:After stimulation of IL-1β, the mRNA and protein expression of TRPA1 in ATDC5 cells were significantly increased (P<0.05). Compared with control blank group, the decreased cell viability, increased IL-6 and TNF-α level,upregulated mRNA level of MMP-13 and ADAMTS5,and downregulated mRNA and protein level of Col Ⅱ and ACAN were observed in IL-1β group (P<0.05). Cell viability and expression of Col Ⅱ and ACAN were obviously increased in IL-1β+siTRPA1 group, whereas the level of IL-6, TNF-α, MMP-13 and ADAMTS5 was markedly decreased in IL-1β+siTRPA1 group compared with IL-1β group (P<0.05). Furthermore, TRPA1 silencing inhibited the increased protein expression of p-p38 MAPK induced by IL-1β (P<0.05).Conclusion:TRPA1 silencing inhibits chondrocyte inflammation and cartilage degeneration induced by IL-1β, which may be through blocking p38 MAPK signaling pathway.
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