Objective:To analyze and discuss the clinical value of HPV and serum folate detection in the evaluation of the outcome of grade Ⅲ cervical intraepithelial neoplasia. Methods:A total of 89 patients with grade Ⅲ cervical intraepithelial neoplasia diagnosed in our hospital from January 2015 to January 2017 were included in the study. Analysis and discussion of HPV and serum folic acid levels were performed to evaluate patients with grade Ⅲ cervical intraepithelial neoplasia. The clinical value of the outcome were analyzed by SPSS 17.0 statistical software package to evaluated the prognosis.Results:The folic acid and HPV viral load distributions of the subjects in the study were as follows; the average folate level in the patients was (25.86±5.96) nmol/L; the folic acid deficiency level was less than 7 nmol/L in serum, and the total folic acid was seen in the patients. The deficiency rate was 41.57%; among them, the proportion of patients with folic acid deficiency in the <35-year-old group was 64.00% and 32.81% in the 35-year-old group. There was a statistically significant difference between the two age groups, P<0.05. The proportion of patients with HPV negative viral load was 17.98%. The proportion of patients with low and medium-low load was 26.97% and 28.09%. The proportion of patients with medium-high load was 21.35%, and the proportion of patients with high load was 5.62%. The difference was not statistically significant among patients of different age groups, P<0.05. Compared with the non-folate-deficient group, patients with folic acid deficiency showed a significantly lower percentage of patients who had subsided and sustained disease prognosis than non-folate-deficient patients. The proportion of malignant transformation was higher in folic acid deficiency patients than in patients without folic acid deficiency, and the difference was statistically significant. P<0.05; the proportion of patients with malignant transformation in the folic acid deficiency group was the highest, and the proportion of patients with non-folate deficiency was the highest, and the difference was statistically significant (P<0.05). Differences in different HPV viral load groupswere statistically significant(χ²=25.89, P<0.05); tumor regression, persistence, progression, and malignant outcomes were compared among HPV-negative, low-, middle-, and mid-and high-load and high-load patients, respectively. The difference between the two groups showed that the proportion of patients with negative load in patients with stable disease was the highest, and the difference between the outcomes was statistically significant (P<0.05). The patients in the middle-high and high-load groups showed progression of the disease. The proportion of patients was the highest, and the difference between the outcome components was statistically significant (P<0.05). The proportion of patients with HPV low-load and low-to-medium load was significant. Conclusion:HPV load and serum folate level have a potential impact on the outcome of patients with grade Ⅲ cervical intraepithelial neoplasia. Folic acid deficiency and high HPV load are associated with poor prognosis.