促红细胞生成素联合甲强龙治疗颈脊髓缺血-再灌注损伤效果分析-现代医学

促红细胞生成素联合甲强龙治疗颈脊髓缺血-再灌注损伤效果分析

单位：1. 湖北医药学院附属襄阳市第一人民医院骨科, 湖北襄阳 441000;
2. 十堰市人民医院内分泌科, 湖北十堰 442000

分类号：R651.2

出版年·卷·期（页码）：2019·47·第八期（960-965）

摘要：

目的：探讨促红细胞生成素（EPO）联合甲强龙治疗颈脊髓缺血-再灌注损伤的效果。方法：研究时间为2015年2月到2018年1月，健康雄性Wistar大鼠30只分成模型组10只、甲强龙组10只与EPO组10组，所有大鼠都建立了颈脊髓缺血-再灌注损伤模型，甲强龙组在脊髓缺血后30 min给予甲强龙静脉注射处理，EPO组在甲强龙组处理的给予静脉注射EPO处理，记录大鼠的神经行为学评分与脊髓损伤情况。结果：所有大鼠均于术后2 h苏醒，EPO组术后7 d、14 d和28 d的Tarlov评分高于甲强龙组与模型组，甲强龙组高于模型组，差异有统计学意义（P<0.05）。术后28 d EPO组脊髓组织的SOD含量高于甲强龙组与模型组，MDA含量低于甲强龙组与模型组，差异有统计学意义（P<0.05），甲强龙组与模型组差异也有统计学意义（P<0.05）。三组的正常运动神经元数目与运动神经元异常率差异有统计学意义（P<0.05）。术后28 d EPO组的IL-6和TNF-α值低于甲强龙组与模型组，甲强龙组低于模型组，差异有统计学意义（P<0.05）。结论：EPO联合甲强龙治疗颈脊髓缺血-再灌注损伤能抑制炎症因子的释放，调节体内氧化/抗氧化的平衡，从而促进脊髓神经功能恢复正常。

Objective:To investigate the effect of erythropoietin (EPO) combined with methylprednisolone on cervical spinal cord ischemia-reperfusion injury. Methods:From February 2015 to January 2018, 30 healthy male Wistar rats were divided into 10 rats in model group, 10 rats in methylprednisolone group and 10 rats in EPO group. All rats were established intomodel of cervical spinal cord ischemia-reperfusion injury. The methylprednisolone group were injected with 30 min after spinal cord ischemia 30 min, and EPO group were injected EPO combined with methylprednisolone after spinal cord ischemia 30 min the neurological behavioral score and spinal cord injury in were recorded in the rats. Results:All the rats were awakened2 h after operation, and the Tarlov scores of postoperative 7 d, 14 d and 28 d in the EPO group were higher than those in the methylprednisolone group and model group, and they were higherin the methylprednisolone group than those of the model group, and the difference were statistically significantly (P<0.05). The content of SOD in the spinal cord tissue of EPO group at postoperative 28 d was higher than that of the methylprednisolone group and model group, and the content of MDA was lower, thedifference was statistically significant (P<0.05). The difference of the number of normal movement and the abnormal rate of motor neuron in the normal movement compared among the three groups were statistically significant (P<0.05). The values of IL-6 and TNF-α on postoperative 28 d in EPO group were lower than those in the model group and methylprednisolone group, while those in themethylprednisolone group were lower than those in themodel group, and compared the difference was statistically significant (P<0.05). Conclusion:EPO combined with methylprednisoloneinhibits the release of inflammatory factors and regulates the balance of oxidation/antioxidant activity in the body, thus promotes the recovery of spinal nerve function.

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