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支气管哮喘患儿外周血单个核细胞中STAT3、STAT4的表达及其意义
作者:马素丽1  张蕾2  杨芳1 
单位:1. 上海市浦东新区人民医院 儿科, 上海 201299;
2. 上海市健康医学院附属浦东新区人民医院 儿科, 上海 201299
关键词:儿童 支气管哮喘 炎症 肺功能 
分类号:R725.6
出版年·卷·期(页码):2019·47·第七期(765-768)
摘要:

目的:探讨支气管哮喘患儿外周血单个核细胞(PBMC)中信号转导与转录激活因子(STAT)3、STAT4的表达及其意义。方法:选择31例支气管哮喘患儿以及35例健康儿童作为研究对象,检测患儿的肺功能和呼出气一氧化氮(FeNO)水平,比较两组患儿的血清白细胞介素(IL)-6、IL-12及嗜酸粒细胞(EOS)水平,分析PBMC中STAT3、STAT4 mRNA表达情况。结果:与对照组比较,哮喘组患儿的STAT4 mRNA水平明显降低,STAT3 mRNA水平明显升高(P<0.05)。STAT3 mRNA表达水平与肺功能指标、IL-12水平呈负相关,与FeNO、EOS、IL-6水平呈正相关(均P<0.05),STAT4 mRNA表达水平与肺功能指标、IL-12水平呈正相关,与FeNO、EOS、IL-6水平呈负相关(均P<0.05)。结论:STAT3、STAT4参与了儿童支气管哮喘的发生和进展,对哮喘的气道炎症具有一定的调节作用。

Objective:To investigate the expression of signal transduction and activator of transcription(STAT)3, STAT4 in peripheral blood mononuclear cells (PBMC)in children with bronchial asthma and its significance. Methods:Thirty-one children with bronchial asthma and 35 healthy children were selected as study subjects. Pulmonary function and fractional exhaled nitric oxide(FeNO)levels were recorded in both groups. Serum levels of interleukin(IL)-6, IL-12, and eosinophilic cells (EOS) were compared between the two groups. The expression of STAT3 mRNA and STAT4 mRNA in PBMC was analyzed. Results:Compared with the control group, the level of STAT4 mRNA in the asthmatic group was significantly lower and the STAT3 mRNAwas significantly higher in the asthmatic group (P<0.05). The expression of STAT3 mRNA was negatively correlated with lung function index and IL-12 level, and positively correlated with FeNO, EOS and IL-6level (all P<0.05). The expression of STAT4 mRNA was positively correlated with lung function index and IL-12level, and was negative correlated with FeNO,EOS and IL-6level (all P<0.05). Conclution:STAT3 and STAT4 are involved in the occurrence and progression of bronchial asthma in children and have a certain regulatory effect on airway inflammation in asthma.

参考文献:

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[3] 隋博文,李明虎,翟平平,等.射干麻黄汤对哮喘小鼠模型气道炎症及血清IL-6、IL-10水平的影响[J].中国中医急症,2017,26(5):783-785,822.
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[6] HAGBERG N,JOELSSON M,LEONARD D,et al.The STAT4 SLE risk allele rs7574865[T] is associated with increased IL-12-induced IFN-γ production in T cells from patients with SLE[J].Ann Rheum Dis,2018,77(7):1070-1077.

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