Objective:To explore the changes and diagnostic value of CD14, CD64, and IL-6 in secondary liver failure. Method:30 patients with secondary liver infection who were hospitalized in our hospital from July 2016 to July 2017 were selected as the infection group.30 patients with hepatic failure treated in our hospital during the same period were selected as uninfected groups. 10 cases of physical examinations in our hospital during the same period for health monitoring were selected as the control group. Patients with confirmed liver failure secondary to ascites infection were treated with drugs; ascites and fasting peripheral venous blood were collected. The levels of CD14 and CD64 in patients were measured using a FACSCalibur flow cytometer produced by BD, USA; IL-6 in serum was measured by an electrochemiluminescence immunoassay(ELISA). Results:The levels of CD14, CD64, and IL-6 in the blood of infected patients(before treatment) were(46.32±8.41) mg·L-1,(12 942.21±328.18) mol/cell,(2 148.13±894.35) pg·dl-1,significantly higher than those of the uninfected group (0.62±0.20) mg·L-1,(1 028.45±139.41) mol/cell,(84.42±56.26) pg·dl-1, and the control group (0.52±0.11) mg·L-1,(983.95±126.27) mol/cell,(13.52±12.71) pg·dl-1, The levels of CD14, CD64, and IL-6 in the ascitic fluid of the infected group(before treatment) were (61.24±6.41) mg·L-1,(14 815.06±362.21) mol/cell, and(9 720.82±7 910.25) pg·dl-1, respectively. In the uninfected group (1.92±1.20) mg·L-1,(836.13±103.22) mol/cell,(1 648.31±1 321.45) pg·dl-1, the difference was statistically significant (P<0.05).The levels of CD14, CD64, and IL-6 in the peripheral blood of the infected patients were (46.32±8.41) mg·L-1,(12 942.21±328.18) mol/cell, and (2 148.13±894.35) pg·dl-1, respectively. Lowere than those in the ascites, CD14, CD64, IL-6 content (61.24±6.41) mg·L-1,(14 815.06±362.21) mol/cell,(9 720.82±7 910.25) pg/dl, the difference between the data was statistically significant (P<0.05). Conclusion:CD 14, CD64 and IL-6 have some reference value in the diagnosis of liver failure caused by infection, and the sensitivity of ascites examination is better than that of serum.
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