川崎病患儿外周血淋巴细胞亚群变化及其临床意义-现代医学

川崎病患儿外周血淋巴细胞亚群变化及其临床意义

单位：1. 安徽省儿童医院急救中心, 安徽合肥 230000;
2. 安徽省儿童医院检验科, 安徽合肥 230000;
3. 安徽省儿童医院消化内科, 安徽合肥 230000

分类号：R446.1

出版年·卷·期（页码）：2019·47·第一期（9-12）

摘要：

目的：探究川崎病（Kawasaki disease，KD）患儿外周血淋巴细胞亚群的变化及其临床意义。方法：选取我院2014年5月至2016年3月收治的川崎病患儿70例作为KD组，112例细菌感染性疾病患儿作为细菌感染组，44例病毒感染性疾病患儿作为病毒感染组，采用流式细胞术对3组患儿外周血淋巴细胞亚群（CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺、CD64⁺）、B细胞、NK细胞和C反应蛋白（CRP）进行检测，比较3组检测结果。结果：外周血CD4⁺、CD4⁺/CD8⁺、B细胞、CRP水平KD组显著高于细菌感染组和病毒感染组，而CD8⁺和NK细胞水平则显著低于细菌感染组和病毒感染组，差异均有统计学意义（P<0.05）；3组间CD3⁺差异无统计学意义；KD患儿CD4⁺、CD8⁺、CD4⁺/CD8⁺值和CD64⁺的受试者工作特征（ROC）曲线下面积分别为0.781、0.725、0.714和0.697。结论：儿童川崎病在急性期往往伴有免疫紊乱及免疫活化情况，CD4⁺、CD8⁺、CD4⁺/CD8⁺值、B细胞、NK细胞和CRP可作为川崎病的早期诊断指标。

Objective: To explore the change of peripheral blood lymphocytes subpopulation in children with Kawasaki disease(KD) and its clinical significance. Methods: A total of 70 KD children (KD group), 112 bacterial infection children (bacterial infection group) and 44 virus infection children (virus infection group)treated in our hospitals from May 2014 to Mar.2016 were enrolled in this study.The number of subpopulation of lymphocytes (CD3⁺, CD4⁺, CD8⁺, CD4⁺/CD8⁺ and CD64⁺), B cells, NK cells and C-reaction protein (CRP) in the 3 groups were detected and compared. Results: Compared with bacterial and virus infection groups,the levels of CD4⁺, CD4⁺/CD8⁺,B cells and CRP in the KD group were significantly increased and the levels of CD8⁺ and NK cells were significantly decreased (all P<0.05);there was no difference for CD3⁺ among the 3 groups. ROCarea of CD4⁺、CD8⁺、CD4⁺/CD8⁺ and CD64⁺ in KD children were 0.781、0.725、0.714 and 0.697, respectively.Conclusions: The acute phase of KD in children is often accompanied by immune disorders and immune activation, CD4⁺,CD8⁺, CD4⁺/CD8⁺, B cells, NK cells and CRP can beused asindicators forearly diagnosis of KD.

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