Objective:To study the change and clinical significance of double-negative T-cells (DNT) in peripheral T lymphocyte subsets of patients with acute exacerbation of bronchial asthma. Methods:A total of 101 cases of patients with acute exacerbation of bronchial asthma admitted to our hospital from April 2015 to November 2017 were selected as asthma group, which was subdivided into mild group, moderate group, severe group and critical group according to the degree of asthmatic attack, and 90 healthy cases in the corresponding period were selected as control group. FC500 flow cytometry (FCM) was adopted to detect the expression level of DNT cells in the peripheral blood of the objects, and on the data were statistically analyzed. Results:DNT cell quantity of patients in the control group was significantly higher than that of acute exacerbation of bronchial asthma (P<0.05), and the difference was statistically significant; DNT cell quantity of patients in severe group was significantly lower than that in mild group and moderate group (P< 0.05), and the difference was statistically significant. Conclusion:The expression of DNT cells in peripheral blood of patients with acute exacerbation of bronchial asthma is low, and the degree of low expression of DNT cells is correlated with the severity of acute attack of bronchial asthma, suggesting that the low expression of DNT cells in peripheral blood playssomepathophysiological effect in acute exacerbation of bronchial asthma, and the severity of acute exacerbation of bronchial asthma can be determined by measuring the expression level of DNT cells. |