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miR-193a和IL17RD在甲状腺癌中的表达及其临床意义
作者:刘美宏  毕洁领  唐文 
单位:宜宾市第二人民医院 乳腺甲状腺科, 四川 宜宾 644000
关键词:甲状腺癌 微小RNA-193a 白细胞介素17受体D 
分类号:R736.1
出版年·卷·期(页码):2018·46·第七期(765-769)
摘要:

目的:探讨微小RNA-193a (miR-193a)和白细胞介素17受体D (IL17RD)在甲状腺癌组织中的表达及其与甲状腺癌临床病理特征的关系。方法:收集经病理学确诊为甲状腺癌(88例)、甲状腺腺瘤(31例)、桥本甲状腺炎(26例)、结节性甲状腺肿(12例)及正常甲状腺(18例)的冷冻组织,采用蛋白免疫印迹、实时定量PCR分析组织中miR-193a和IL17RD mRNA及其蛋白表达情况,并分析它们与甲状腺癌临床病理特征的关系。结果:与正常甲状腺组织相比,桥本甲状腺炎、甲状腺腺瘤、甲状腺癌组织中miR-193a表达水平显著降低,而IL17RD mRNA和蛋白表达显著增加(均P<0.05)。甲状腺癌组织中miR-193a和IL17RD蛋白表达水平与肿瘤大小、临床分期、组织学分化程度及淋巴结转移等临床特征有关(均P<0.05)。miR-193a与IL17RD mRNA和蛋白表达均呈明显负相关(P<0.05)。结论:甲状腺癌组织中miR-193a和IL17RD的表达与肿瘤大小、临床分期、组织学分化、淋巴结转移等特征有关,miR-193a可能通过靶向IL17RD参与甲状腺癌的发生及进展。

Objective: To investigate the expression of miR-193a and IL17RD in thyroid carcinoma, and analyze the relationship with clinicopathological features of thyroid carcinoma. Methods: The frozen tissues from pathologically confirmed thyroid cancer (88 cases), thyroid adenoma (31 cases), Hashimoto's thyroiditis (26 cases), nodular goiter (12 cases) and normal thyroid gland (18 cases) were collected. Western blot, real-time quantitative PCR were used to analyze the expression of miR-193a and IL17RD in the tissues. The relationship with clinicopathological features of thyroid carcinoma was explored. Results: Compared with normal thyroid tissue, the expression of miR-193a in Hashimoto's thyroiditis, thyroid adenoma and thyroid cancer tissue were significantly decreased, while the expression of IL17RD was significantly increased (all P<0.05).The expression of miR-193a and IL17RD protein in thyroid cancer tissue were related to the clinical features such as tumor size,clinical stage,histological grade and lymph node metastasis(P<0.05).There was a significant negative correlation between the expression of miR-193a and IL17RD (P<0.05). Conclusion: The expressionof miR-193a and IL17RD in thyroid cancer tissues are related to tumor size,clinical stage,histological differentiation, and lymph node metastasis.miR-193a may be involved in the progression of thyroid cancer by targeting IL17RD.

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