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rmhTNF-α抑制胃癌细胞增殖作用机制的研究
作者:张春平1  代洪生1  王津迪1  潘光敏1  高志星2  季万胜2 
单位:1. 潍坊医学院, 山东 潍坊 261053;
2. 潍坊医学院附属医院 消化内科, 山东 潍坊 261031
关键词:Δ133p53 cjun cfos 重组改构人肿瘤坏死因子-α 胃癌细胞 增殖 
分类号:R735.2
出版年·卷·期(页码):2018·46·第七期(748-750)
摘要:

目的:探讨重组改构人肿瘤坏死因子-α(rmhTNF-α)抑制人胃癌细胞增殖的作用机制。方法:将不同浓度(0、50、100、200 U·ml-1)的rmhTNF-α作用于人胃癌细胞系MKN45(干预组),采用增殖/毒性检测试剂(CCK-8)测定细胞抑制率,实时荧光定量PCR测定Δ133p53、cjun、cfos mRNA表达变化。结果:细胞抑制率干预组高于对照组(rmhTNF-α 0 U·ml-1组),且随rmhTNF-α浓度的增加而增高(P<0.05);Δ133p53 mRNA表达干预组较对照组低,且随rmhTNF-α浓度的增加而降低(P<0.05),cjun、cfos mRNA表达干预组较对照组高,且随rmhTNF-α浓度的增加而增高(均P<0.05)。Pearson相关分析显示,Δ133p53 mRNA与cjun和cfos mRNA的表达呈显著负相关(分别r=-0.954、-0.947,均P<0.01)。结论:rmhTNF-α可能是通过抑制Δ133p53表达并促进cjun、cfos表达而抑制胃癌细胞的增殖,其对Δ133p53的抑制作用可能是cjun、cfos介导的。

Objective: To investigate the mechanism of recombinant mutant human tumor necrosis factor-α(rmhTNF-α) inhibit the proliferation of human gastric cancer cell. Methods: Human gastric cancer cell lines MKN45 were treated with different concentrations(0, 50,100,200 U·ml-1) of rmhTNF-α intervention group.The cell inhibition rate was detected by proliferation/toxicity detection kit (CCK-8), and the expression of Δ133p53,cjun,cfos mRNA were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction. Results: The cell inhibition rates in the intervention group was higher than that in the control group(rmhTNF-α 0 U·ml-1 group)and it increased with the increasing of concentration of rmhTNF-α(P<0.05); the expression of Δ133p53 mRNA in the intervention group was lower than that in the control group and it decreased with the increasing of concentration of rmhTNF-α(P<0.05);the expression of cjun,cfos mRNA in the intervention group were higher than those in the control group and they increased with the increasing of concentration of rmhTNF-α(all P<0.05). Pearson correclation analysis showed the expression was negatively correlated markedly betweenΔ133p53 mRNA and cjun,cfos mRNA(r=-0.954,-0.947,respectivly,all P<0.01). Conclusion: rmhTNF may inhibit Δ133p53 expression and promote cjun,cfos expression to further inhibit gastric cancer cell proliferation,the inhibitory effect on Δ133p53 may mediated by cjun and cfos.

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